biostudies-literatureCurvino EJNIBIB NIH HHSNIAID NIH HHSNational Science Foundation (NSF)628-649https://www.ebi.ac.uk/biostudies/studies/S-EPMC11128482biostudies-literatureUnknown8(5)Inflammatory bowel disease lacks a long-lasting and broadly effective therapy. Here, by taking advantage of the anti-infection and anti-inflammatory properties of natural antibodies against the small-molecule epitope phosphorylcholine (PC), we show in multiple mouse models of colitis that immunization of the animals with self-assembling supramolecular peptide nanofibres bearing PC epitopes induced sustained levels of anti-PC antibodies that were both protective and therapeutic. The strength and type of immune responses elicited by the nanofibres could be controlled through the relative valency of PC epitopes and exogenous T-cell epitopes on the nanofibres and via the addition of the adjuvant CpG. The nanomaterial-assisted induction of the production of therapeutic antibodies may represent Nature biomedical engineeringEngaging natural antibody responses for the treatment of inflammatory bowel disease via phosphorylcholine-presenting nanofibres.PMC11128482R01 EB009701R01 AI172151DGE-1644868Collier JHCurvino EJSegura TWoodruff MEFreire Haddad HVotaw NLHale LPAnderson ARRoe EFfalseEngaging natural antibody responses for the treatment of inflammatory bowel disease via phosphorylcholine-presenting nanofibres.Inflammatory bowel disease lacks a long-lasting and broadly effective therapy. Here, by taking advantage of the anti-infection and anti-inflammatory properties of natural antibodies against the small-molecule epitope phosphorylcholine (PC), we show in multiple mouse models of colitis that immunization of the animals with self-assembling supramolecular peptide nanofibres bearing PC epitopes induced sustained levels of anti-PC antibodies that were both protective and therapeutic. The strength and type of immune responses elicited by the nanofibres could be controlled through the relative valency of PC epitopes and exogenous T-cell epitopes on the nanofibres and via the addition of the adjuvant CpG. The nanomaterial-assisted induction of the production of therapeutic antibodies may represent 2024-01-01T00:00:00Z2024 May2025-04-04T13:19:33.082Z2025-04-04T13:19:33.082ZS-EPMC111284823801230810.1038/s41551-023-01139-6