biostudies-literatureWang TJiangsu Agricultural Science and Technology Innovation FundOpen Funding of the Xuyi Center of Attapulgite Applied Technology Research Development &amp; IndustrializationNational Key R&amp;D Programhe Guidance Foundation, the Sanya Institute of Nanjing Agricultural University Research120https://www.ebi.ac.uk/biostudies/studies/S-EPMC11137985biostudies-literatureUnknown21(1)<h4>Background</h4>The Porcine Epidemic Diarrhea Virus (PEDV) has caused significant economic losses in the global swine industry. As a potential drug for treating diarrhea, the antiviral properties of attapulgite deserve further study.<h4>Methods</h4>In this study, various methods such as RT-qPCR, Western blot, viral titer assay, Cytopathic Effect, immunofluorescence analysis and transmission electron microscopy were used to detect the antiviral activity of attapulgite and to assess its inhibitory effect on PEDV.<h4>Results</h4>When exposed to the same amount of virus, there was a significant decrease in the expression of the S protein, resulting in a viral titer reduction from 10^-5.613 TCID₅₀/mL to 10^-2.90 TCID₅₀/mL, which represents a decreaseVirology journalVirological evaluation of natural and modified attapulgite against porcine epidemic diarrhoea virus.PMC111379852021YFD1800500CX(21)2038NAUSY-ZD08Wen YXue FWang AWang TZhang XQian BXu XZhou YTang FDai JfalseVirological evaluation of natural and modified attapulgite against porcine epidemic diarrhoea virus.<h4>Background</h4>The Porcine Epidemic Diarrhea Virus (PEDV) has caused significant economic losses in the global swine industry. As a potential drug for treating diarrhea, the antiviral properties of attapulgite deserve further study.<h4>Methods</h4>In this study, various methods such as RT-qPCR, Western blot, viral titer assay, Cytopathic Effect, immunofluorescence analysis and transmission electron microscopy were used to detect the antiviral activity of attapulgite and to assess its inhibitory effect on PEDV.<h4>Results</h4>When exposed to the same amount of virus, there was a significant decrease in the expression of the S protein, resulting in a viral titer reduction from 10^-5.613 TCID₅₀/mL to 10^-2.90 TCID₅₀/mL, which represents a decrease2024-01-01T00:00:00Z2024 May2026-06-03T01:32:13.202Z2026-04-22T03:13:29.932ZS-EPMC111379853881673810.1186/s12985-024-02396-w