{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["McCaughey CS"],"funding":["National Institute of Allergy and Infectious Diseases","University of California, Santa Cruz","NIAID NIH HHS","Division of Integrative Organismal Systems","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["8308-8316"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11140670"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["96(21)"],"pubmed_abstract":["Microbial biofilms represent an important lifestyle for bacteria and are dynamic three-dimensional structures. Cyclic dimeric guanosine monophosphate (c-di-GMP) is a ubiquitous signaling molecule that is known to be tightly regulated with biofilm processes. While measurements of global levels of c-di-GMP have proven valuable toward understanding the genetic control of c-di-GMP production, there is a need for tools to observe the local changes of c-di-GMP production in biofilm processes. We have developed a label-free method for the direct detection of c-di-GMP in microbial colony biofilms using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). We applied this method to the enteric pathogen <i>Vibrio cholerae</i>, the marine symbiont <i>V. fischeri</i>, and "],"journal":["Analytical chemistry"],"pubmed_title":["A Label-Free Approach for Relative Spatial Quantitation of c-di-GMP in Microbial Biofilms."],"pmcid":["PMC11140670"],"funding_grant_id":["R35GM148385","R01 AI102584","R01AI102584","2220511","2220510","R35 GM148385","K12 GM119955"],"pubmed_authors":["Sanchez LM","Yildiz FH","Mandel MJ","McCaughey CS","Isenberg RY","Trebino MA","McAtamney A"],"additional_accession":[]},"is_claimable":false,"name":"A Label-Free Approach for Relative Spatial Quantitation of c-di-GMP in Microbial Biofilms.","description":"Microbial biofilms represent an important lifestyle for bacteria and are dynamic three-dimensional structures. Cyclic dimeric guanosine monophosphate (c-di-GMP) is a ubiquitous signaling molecule that is known to be tightly regulated with biofilm processes. While measurements of global levels of c-di-GMP have proven valuable toward understanding the genetic control of c-di-GMP production, there is a need for tools to observe the local changes of c-di-GMP production in biofilm processes. We have developed a label-free method for the direct detection of c-di-GMP in microbial colony biofilms using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). We applied this method to the enteric pathogen <i>Vibrio cholerae</i>, the marine symbiont <i>V. fischeri</i>, and ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-03T03:58:41.004Z","creation":"2025-08-14T03:05:50.211Z"},"accession":"S-EPMC11140670","cross_references":{"pubmed":["38752543"],"doi":["10.1021/acs.analchem.3c04687"]}}