biostudies-literatureUnknown118(2)Sadeghi PIsfahan University of Medical Sciences<i>Pseudomonas aeruginosa</i> has different antibiotic resistance pathways, such as broad-spectrum lactamases and metallo-β-lactamases (MBL), penicillin-binding protein (PBP) alteration, and active efflux pumps. Polymerase chain reaction (PCR) and sequencing methods were applied for double-locus sequence typing (DLST) and New Delhi metallo-β-lactamase (NDM) typing. We deduced the evolutionary pathways for DLST and NDM genes of P. aeruginosa using phylogenetic network. Among the analyzed isolates, 62.50% of the <i>P. aeruginosa</i> isolates were phenotypically carbapenem resistance (CARBR) isolates. Characterization of isolates revealed that the prevalence of bla_NDM, bla_VIM, bla_IMP, undetermined carbapenemase, and MexAB-OprM were 27.5%, 2%, 2.5%, 12.5%, and Pathogens and global health170-181https://www.ebi.ac.uk/biostudies/studies/S-EPMC11141312biostudies-literatureEvolutionary trajectories of beta-lactamase NDM and DLST cluster in <i>Pseudomonas aeruginosa</i>: finding the putative ancestor.PMC11141312Salari-Jazi AAspatwar AMahnam KFaghri JSadeghi PfalseEvolutionary trajectories of beta-lactamase NDM and DLST cluster in <i>Pseudomonas aeruginosa</i>: finding the putative ancestor.<i>Pseudomonas aeruginosa</i> has different antibiotic resistance pathways, such as broad-spectrum lactamases and metallo-β-lactamases (MBL), penicillin-binding protein (PBP) alteration, and active efflux pumps. Polymerase chain reaction (PCR) and sequencing methods were applied for double-locus sequence typing (DLST) and New Delhi metallo-β-lactamase (NDM) typing. We deduced the evolutionary pathways for DLST and NDM genes of P. aeruginosa using phylogenetic network. Among the analyzed isolates, 62.50% of the <i>P. aeruginosa</i> isolates were phenotypically carbapenem resistance (CARBR) isolates. Characterization of isolates revealed that the prevalence of bla_NDM, bla_VIM, bla_IMP, undetermined carbapenemase, and MexAB-OprM were 27.5%, 2%, 2.5%, 12.5%, and 2024-01-01T00:00:00Z2024 Mar2025-04-26T08:04:12.324Z2025-04-06T12:35:05.561ZS-EPMC111413123746488410.1080/20477724.2023.2236416