{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lehman SS"],"funding":["National Institute of Allergy and Infectious Diseases","Intramural NIH HHS","Eunice Kennedy Shriver National Institute of Child Health and Human Development","National Heart, Lung, and Blood Institute","National Institutes of Health"],"pagination":["114033"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11141579"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["43(4)"],"pubmed_abstract":["Small GTPases of the Ras subfamily are best known for their role as proto-oncoproteins, while their function during microbial infection has remained elusive. Here, we show that Legionella pneumophila hijacks the small GTPase NRas to the Legionella-containing vacuole (LCV) surface. A CRISPR interference screen identifies a single L. pneumophila effector, DenR (Lpg1909), required for this process. Recruitment is specific for NRas, while its homologs KRas and HRas are excluded from LCVs. The C-terminal hypervariable tail of NRas is sufficient for recruitment, and interference with either NRas farnesylation or S-acylation sites abrogates recruitment. Intriguingly, we detect markers of active NRas signaling on the LCV, suggesting it acts as a signaling platform. Subsequent phosphoproteomics ana"],"journal":["Cell reports"],"pubmed_title":["The Legionella pneumophila effector DenR hijacks the host NRas proto-oncoprotein to downregulate MAPK signaling."],"pmcid":["PMC11141579"],"funding_grant_id":["ZIA HD008893","1 ZIA HD008893-12","1 ZIA AI001085-13"],"pubmed_authors":["Tucholski T","Williamson CD","Allen M","Bouchard S","Jarnik M","Machner MP","Lehman SS","Ellis NA","Nita-Lazar A"],"additional_accession":[]},"is_claimable":false,"name":"The Legionella pneumophila effector DenR hijacks the host NRas proto-oncoprotein to downregulate MAPK signaling.","description":"Small GTPases of the Ras subfamily are best known for their role as proto-oncoproteins, while their function during microbial infection has remained elusive. Here, we show that Legionella pneumophila hijacks the small GTPase NRas to the Legionella-containing vacuole (LCV) surface. A CRISPR interference screen identifies a single L. pneumophila effector, DenR (Lpg1909), required for this process. Recruitment is specific for NRas, while its homologs KRas and HRas are excluded from LCVs. The C-terminal hypervariable tail of NRas is sufficient for recruitment, and interference with either NRas farnesylation or S-acylation sites abrogates recruitment. Intriguingly, we detect markers of active NRas signaling on the LCV, suggesting it acts as a signaling platform. Subsequent phosphoproteomics ana","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-06-02T01:41:53.733Z","creation":"2026-04-13T03:11:58.808Z"},"accession":"S-EPMC11141579","cross_references":{"pubmed":["38568811"],"doi":["10.1016/j.celrep.2024.114033"]}}