biostudies-literatureWen ZNIDCR NIH HHSNIAID NIH HHSNational Cancer InstituteNCI NIH HHSNational Institutes of HealthWelch FoundationUT Southwestern Medical CenterNIGMS NIH HHSCancer Prevention and Research Institute of Texas100398https://www.ebi.ac.uk/biostudies/studies/S-EPMC11141889biostudies-literatureUnknown37(2)Immunohistochemistry (IHC) is a well-established and commonly used staining method for clinical diagnosis and biomedical research. In most IHC images, the target protein is conjugated with a specific antibody and stained using diaminobenzidine (DAB), resulting in a brown coloration, whereas hematoxylin serves as a blue counterstain for cell nuclei. The protein expression level is quantified through the H-score, calculated from DAB staining intensity within the target cell region. Traditionally, this process requires evaluation by 2 expert pathologists, which is both time consuming and subjective. To enhance the efficiency and accuracy of this process, we have developed an automatic algorithm for quantifying the H-score of IHC images. To characterize protein expression in specific cell regiModern pathology : an official journal of the United States and Canadian Academy of Pathology, IncDeep Learning-Based H-Score Quantification of Immunohistochemistry-Stained Images.PMC111418891U01CA249245F99 CA253718I-1881R01 DE0306561R01GM140012P30 CA142543R01CA273585-01A1P30CA142543P50 CA070907RP230330R01 CA273585R01CA2077631R01DE030656RP190610U01 CA249245K00CA253718RP200327R01 GM141519RP180805R01GM115473R01 GM1154731R01GM141519U01 AI156189R35 GM136375P50CA70907R01 GM140012R01 CA207763Solis Soto LMWen ZBrekken RADaoud EVXie YWistuba IIYang DMGu ZFujimoto JJia LBehrens CLuo DFang YEvers BMArner ENRong RWang SO'Donnell KALewis CMXiao GYang SfalseDeep Learning-Based H-Score Quantification of Immunohistochemistry-Stained Images.Immunohistochemistry (IHC) is a well-established and commonly used staining method for clinical diagnosis and biomedical research. In most IHC images, the target protein is conjugated with a specific antibody and stained using diaminobenzidine (DAB), resulting in a brown coloration, whereas hematoxylin serves as a blue counterstain for cell nuclei. The protein expression level is quantified through the H-score, calculated from DAB staining intensity within the target cell region. Traditionally, this process requires evaluation by 2 expert pathologists, which is both time consuming and subjective. To enhance the efficiency and accuracy of this process, we have developed an automatic algorithm for quantifying the H-score of IHC images. To characterize protein expression in specific cell regi2024-01-01T00:00:00Z2024 Feb2026-03-12T23:06:54.016Z2025-08-12T03:05:01.689ZS-EPMC111418893804378810.1016/j.modpat.2023.100398