{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mouwenda YD"],"funding":["DZIF","European & Developing Countries Clinical Trials Partnership (EDCTP)","NIAID NIH HHS","Dutch Research Council (NWO)","NIH","WISE"],"pagination":["e170210"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11141902"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(9)"],"pubmed_abstract":["Vaccination of malaria-naive volunteers with a high dose of Plasmodium falciparum sporozoites chemoattenuated by chloroquine (CQ) (PfSPZ-CVac [CQ]) has previously demonstrated full protection against controlled human malaria infection (CHMI). However, lower doses of PfSPZ-CVac [CQ] resulted in incomplete protection. This provides the opportunity to understand the immune mechanisms needed for better vaccine-induced protection by comparing individuals who were protected with those not protected. Using mass cytometry, we characterized immune cell composition and responses of malaria-naive European volunteers who received either lower doses of PfSPZ-CVac [CQ], resulting in 50% protection irrespective of the dose, or a placebo vaccination, with everyone becoming infected following CHMI. Clusters of CD4+ and γδ T cells associated with protection were identified, consistent with their known role in malaria immunity. Additionally, EMRA CD8+ T cells and CD56+CD8+ T cell clusters were associated with protection. In a cohort from a malaria-endemic area in Gabon, these CD8+ T cell clusters were also associated with parasitemia control in individuals with lifelong exposure to malaria. Upon stimulation with P. falciparum-infected erythrocytes, CD4+, γδ, and EMRA CD8+ T cells produced IFN-γ and/or TNF, indicating their ability to mediate responses that eliminate malaria parasites."],"journal":["JCI insight"],"pubmed_title":["Immune responses associated with protection induced by chemoattenuated PfSPZ vaccine in malaria-naive Europeans."],"pmcid":["PMC11141902"],"funding_grant_id":["R44 AI058375","U01 AI165745","U01A165745","5R44AI055229","5R44AI058375","SPI.2021.004","DZIF TTU 03.802","R44 AI055229","RIA2018SV-2310","PSIA2020AGDG-3316"],"pubmed_authors":["Betouke Ongwe ME","Fendel R","Mordmuller B","Esen M","Stam KA","Mouwenda YD","Jochems SP","Yazdanbakhsh M","Massinga Loembe M","Hoffman SL","Van Unen V","Kremsner PG","Sim BKL","de Steenhuijsen Piters WAA"],"additional_accession":[]},"is_claimable":false,"name":"Immune responses associated with protection induced by chemoattenuated PfSPZ vaccine in malaria-naive Europeans.","description":"Vaccination of malaria-naive volunteers with a high dose of Plasmodium falciparum sporozoites chemoattenuated by chloroquine (CQ) (PfSPZ-CVac [CQ]) has previously demonstrated full protection against controlled human malaria infection (CHMI). However, lower doses of PfSPZ-CVac [CQ] resulted in incomplete protection. This provides the opportunity to understand the immune mechanisms needed for better vaccine-induced protection by comparing individuals who were protected with those not protected. Using mass cytometry, we characterized immune cell composition and responses of malaria-naive European volunteers who received either lower doses of PfSPZ-CVac [CQ], resulting in 50% protection irrespective of the dose, or a placebo vaccination, with everyone becoming infected following CHMI. Clusters of CD4+ and γδ T cells associated with protection were identified, consistent with their known role in malaria immunity. Additionally, EMRA CD8+ T cells and CD56+CD8+ T cell clusters were associated with protection. In a cohort from a malaria-endemic area in Gabon, these CD8+ T cell clusters were also associated with parasitemia control in individuals with lifelong exposure to malaria. Upon stimulation with P. falciparum-infected erythrocytes, CD4+, γδ, and EMRA CD8+ T cells produced IFN-γ and/or TNF, indicating their ability to mediate responses that eliminate malaria parasites.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-01T11:58:35.556Z","creation":"2026-04-08T12:09:20.118Z"},"accession":"S-EPMC11141902","cross_references":{"pubmed":["38716733"],"doi":["10.1172/jci.insight.170210"]}}