{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Basta DW"],"funding":["NIDDK NIH HHS","NIAID NIH HHS"],"pubmed_abstract":["Transposon insertion sequencing (Tn-seq) is a powerful method for genome-scale functional genetics in bacteria. However, its effectiveness is often limited by a lack of mutant diversity, caused by either inefficient transposon delivery or stochastic loss of mutants due to population bottlenecks. Here, we introduce \"InducTn-seq\", which leverages inducible mutagenesis for temporal control of transposition. InducTn-seq generates millions of transposon mutants from a single colony, enabling the sensitive detection of subtle fitness defects and transforming binary classifications of gene essentiality into a quantitative fitness measurement across both essential and non-essential genes. Using a mouse model of infectious colitis, we show that InducTn-seq bypasses a highly restrictive host bottlen"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.05.21.595064"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11142078"],"repository":["biostudies-literature"],"pubmed_title":["Inducible transposon mutagenesis for genome-scale forward genetics."],"pmcid":["PMC11142078"],"funding_grant_id":["F31 AI156949","R01 AI042347","T32 DK007477","P30 DK034854"],"pubmed_authors":["Waldor MK","Basta DW","Hotinger JA","Hullahalli K","Sullivan EJ","Campbell IW"],"additional_accession":[]},"is_claimable":false,"name":"Inducible transposon mutagenesis for genome-scale forward genetics.","description":"Transposon insertion sequencing (Tn-seq) is a powerful method for genome-scale functional genetics in bacteria. However, its effectiveness is often limited by a lack of mutant diversity, caused by either inefficient transposon delivery or stochastic loss of mutants due to population bottlenecks. Here, we introduce \"InducTn-seq\", which leverages inducible mutagenesis for temporal control of transposition. InducTn-seq generates millions of transposon mutants from a single colony, enabling the sensitive detection of subtle fitness defects and transforming binary classifications of gene essentiality into a quantitative fitness measurement across both essential and non-essential genes. Using a mouse model of infectious colitis, we show that InducTn-seq bypasses a highly restrictive host bottlen","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-04-15T03:32:54.905Z","creation":"2026-04-15T03:15:39.84Z"},"accession":"S-EPMC11142078","cross_references":{"pubmed":["38826325"],"doi":["10.1101/2024.05.21.595064"]}}