biostudies-literatureHou LYDeutsche Forschungsgemeinschaft1536-1560https://www.ebi.ac.uk/biostudies/studies/S-EPMC11142374biostudies-literatureUnknown195(2)Thiol-based redox regulation is a crucial posttranslational mechanism to acclimate plants to changing light availability. Here, we conducted a biotin switch-based redox proteomics study in Arabidopsis (Arabidopsis thaliana) to systematically investigate dynamics of thiol-redox networks in response to temporal changes in light availability and across genotypes lacking parts of the thioredoxin (Trx) or NADPH-Trx-reductase C (NTRC) systems in the chloroplast. Time-resolved dynamics revealed light led to marked decreases in the oxidation states of many chloroplast proteins with photosynthetic functions during the first 10 min, followed by their partial reoxidation after 2 to 6 h into the photoperiod. This involved f, m, and x-type Trx proteins showing similar light-induced reduction-oxidation Plant physiologyThe impact of light and thioredoxins on the plant thiol-disulfide proteome.PMC11142374TRR175Poeker LSchroda MHou LYGeigenberger PSommer FDziubek DfalseThe impact of light and thioredoxins on the plant thiol-disulfide proteome.Thiol-based redox regulation is a crucial posttranslational mechanism to acclimate plants to changing light availability. Here, we conducted a biotin switch-based redox proteomics study in Arabidopsis (Arabidopsis thaliana) to systematically investigate dynamics of thiol-redox networks in response to temporal changes in light availability and across genotypes lacking parts of the thioredoxin (Trx) or NADPH-Trx-reductase C (NTRC) systems in the chloroplast. Time-resolved dynamics revealed light led to marked decreases in the oxidation states of many chloroplast proteins with photosynthetic functions during the first 10 min, followed by their partial reoxidation after 2 to 6 h into the photoperiod. This involved f, m, and x-type Trx proteins showing similar light-induced reduction-oxidation 2024-01-01T00:00:00Z2024 May2026-05-26T20:10:01.312Z2026-05-26T03:07:16.846ZS-EPMC111423743821404310.1093/plphys/kiad669