{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu QZ"],"funding":["The Science and Technology Commission of Shanghai Hongkou District","\"Special fund for scientific research startup\" by Shanghai Fourth People's Hospital","medical engineering of Shanghai Jiao Tong University"],"pagination":["1749-1762"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11145145"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["115(6)"],"pubmed_abstract":["N<sup>6</sup>-Methyladenosine (m<sup>6</sup>A) is a important process regulating gene expression post-transcriptionally. Programmed death ligand 1 (PD-L1) is a major immune inhibitive checkpoint that facilitates immune evasion and is expressed in tumor cells. In this research we discovered that Wilms' tumor 1-associated protein (WTAP) degradation caused by ubiquitin-mediated cleavage in cancer cells (colorectal cancer, CRC) under hypoxia was inhibited by Pumilio homolog 1 (PUM1) directly bound to WTAP. WTAP enhanced PD-L1 expression in a way that was m<sup>6</sup>A-dependent. m<sup>6</sup>A \"reader,\" Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) identified methylated PD-L1 transcripts and subsequently fixed its mRNA. Additionally, we found that T-cell proliferation and its "],"journal":["Cancer science"],"pubmed_title":["WTAP-induced N<sup>6</sup>-methyladenosine of PD-L1 blocked T-cell-mediated antitumor activity under hypoxia in colorectal cancer."],"pmcid":["PMC11145145"],"funding_grant_id":["sykyqd04401 and sykyqd05101","YG2022QN115","hongwei2202-19"],"pubmed_authors":["Huang ZX","Qiao GL","Zhou MJ","Chen Z","Zhang N","Chen JY","Zhou DH","Tu XH","Xie YX","Liu QZ"],"additional_accession":[]},"is_claimable":false,"name":"WTAP-induced N<sup>6</sup>-methyladenosine of PD-L1 blocked T-cell-mediated antitumor activity under hypoxia in colorectal cancer.","description":"N<sup>6</sup>-Methyladenosine (m<sup>6</sup>A) is a important process regulating gene expression post-transcriptionally. Programmed death ligand 1 (PD-L1) is a major immune inhibitive checkpoint that facilitates immune evasion and is expressed in tumor cells. In this research we discovered that Wilms' tumor 1-associated protein (WTAP) degradation caused by ubiquitin-mediated cleavage in cancer cells (colorectal cancer, CRC) under hypoxia was inhibited by Pumilio homolog 1 (PUM1) directly bound to WTAP. WTAP enhanced PD-L1 expression in a way that was m<sup>6</sup>A-dependent. m<sup>6</sup>A \"reader,\" Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) identified methylated PD-L1 transcripts and subsequently fixed its mRNA. Additionally, we found that T-cell proliferation and its ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-06-02T06:09:15.837Z","creation":"2026-04-15T03:10:06.799Z"},"accession":"S-EPMC11145145","cross_references":{"pubmed":["38508217"],"doi":["10.1111/cas.16136"]}}