biostudies-literatureMonzon-Casanova EWellcome TrustBiotechnology and Biological Sciences Research Council2266-2273https://www.ebi.ac.uk/biostudies/studies/S-EPMC11146436biostudies-literatureUnknown51(9)The maturation of immature B cells and the survival of mature B cells is stringently controlled to maintain a diverse repertoire of antibody specificities while avoiding self-reactivity. At the molecular level this is regulated by signaling from membrane Ig and the BAFF-receptor that sustain a pro-survival program of gene expression. Whether and how posttranscriptional mechanisms contribute to B cell maturation and survival remains poorly understood. Here, we show that the polypyrimidine tract binding proteins (PTBP) PTBP1 and PTBP3 bind to a large and overlapping set of transcripts in B cells. Both PTBP1 and PTBP3 bind to introns and exons where they are predicted to regulate alternative splicing. Moreover, they also show high-density of binding to 3' untranslated regions suggesting they European journal of immunologyEssential requirement for polypyrimidine tract binding proteins 1 and 3 in the maturation and maintenance of mature B cells in mice.PMC11146436BBS/E/B/000C0407BBS/E/B/000C0428200823/Z/16/ZBBS/E/B/000C0427BB/P01898X/1BB/J001457/1Smith CWJMonzon-Casanova ETurner MBates KJfalseEssential requirement for polypyrimidine tract binding proteins 1 and 3 in the maturation and maintenance of mature B cells in mice.The maturation of immature B cells and the survival of mature B cells is stringently controlled to maintain a diverse repertoire of antibody specificities while avoiding self-reactivity. At the molecular level this is regulated by signaling from membrane Ig and the BAFF-receptor that sustain a pro-survival program of gene expression. Whether and how posttranscriptional mechanisms contribute to B cell maturation and survival remains poorly understood. Here, we show that the polypyrimidine tract binding proteins (PTBP) PTBP1 and PTBP3 bind to a large and overlapping set of transcripts in B cells. Both PTBP1 and PTBP3 bind to introns and exons where they are predicted to regulate alternative splicing. Moreover, they also show high-density of binding to 3' untranslated regions suggesting they 2021-01-01T00:00:00Z2021 Sep2026-06-02T06:16:34.224Z2026-04-15T03:15:07.101ZS-EPMC111464363421419210.1002/eji.202149257