{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ares M"],"funding":["National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["322-335"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11146597"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["38(7-8)"],"pubmed_abstract":["Rare, full-length circular intron RNAs distinct from lariats have been reported in several species, but their biogenesis is not understood. We envisioned and tested a hypothesis for their formation using <i>Saccharomyces cerevisiae</i>, documenting full-length and novel processed circular RNAs from multiple introns. Evidence implicates a previously undescribed catalytic activity of the intron lariat spliceosome (ILS) in which the 3'-OH of the lariat tail (with optional trimming and adenylation by the nuclear 3' processing machinery) attacks the branch, joining the intron 3' end to the 5' splice site in a 3'-5' linked circle. Human U2 and U12 spliceosomes produce analogous full-length and processed circles. Postsplicing catalytic activity of the spliceosome may promote intron transposition "],"journal":["Genes & development"],"pubmed_title":["Intron lariat spliceosomes convert lariats to true circles: implications for intron transposition."],"pmcid":["PMC11146597"],"funding_grant_id":["R01 GM040478","R35 GM145266"],"pubmed_authors":["Donohue JP","Igel H","Ares M","Katzman S"],"additional_accession":[]},"is_claimable":false,"name":"Intron lariat spliceosomes convert lariats to true circles: implications for intron transposition.","description":"Rare, full-length circular intron RNAs distinct from lariats have been reported in several species, but their biogenesis is not understood. We envisioned and tested a hypothesis for their formation using <i>Saccharomyces cerevisiae</i>, documenting full-length and novel processed circular RNAs from multiple introns. Evidence implicates a previously undescribed catalytic activity of the intron lariat spliceosome (ILS) in which the 3'-OH of the lariat tail (with optional trimming and adenylation by the nuclear 3' processing machinery) attacks the branch, joining the intron 3' end to the 5' splice site in a 3'-5' linked circle. Human U2 and U12 spliceosomes produce analogous full-length and processed circles. Postsplicing catalytic activity of the spliceosome may promote intron transposition ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2026-06-02T23:22:56.589Z","creation":"2025-04-04T07:40:50.314Z"},"accession":"S-EPMC11146597","cross_references":{"pubmed":["38724209"],"doi":["10.1101/gad.351764.124"]}}