biostudies-literatureChaubal RDepartment of Atomic Energy, Government of IndiaMizuho Bank LimitedAkhil GuptaSunil GuptaDepartment of Atomic Energy, Government of India (DAE)Department of Biotechnology, Ministry of Science and Technology, India (DBT)Department of Biotechnology, Ministry of Science and Technology, IndiaWomens Cancer Initiative572-584https://www.ebi.ac.uk/biostudies/studies/S-EPMC11148542biostudies-literatureUnknown22(6)Surgery exposes tumor tissue to severe hypoxia and mechanical stress leading to rapid gene expression changes in the tumor and its microenvironment, which remain poorly characterized. We biopsied tumor and adjacent normal tissues from patients with breast (n = 81) and head/neck squamous cancers (HNSC; n = 10) at the beginning (A), during (B), and end of surgery (C). Tumor/normal RNA from 46/81 patients with breast cancer was subjected to mRNA-Seq using Illumina short-read technology, and from nine patients with HNSC to whole-transcriptome microarray with Illumina BeadArray. Pathways and genes involved in 7 of 10 known cancer hallmarks, namely, tumor-promoting inflammation (TNF-A, NFK-B, IL18 pathways), activation of invasion and migration (various extracellular matrix-related pathways, celMolecular cancer research : MCRSurgical Tumor Resection Deregulates Hallmarks of Cancer in Resected Tissue and the Surrounding Microenvironment.PMC11148542Research GrantBT/MED/30/VNCI-Hr-BRCA/2015Personal Research Grant for Laboratory Infrastructure from Mr. Akhil GuptaDAE CTC-TMCPersonal Research Grant from Mr. Sunil Gupta Formerly at Tata MotorsCorporate Social Responsibility Research Grant for Institutional InfrastructureDutt ATalker EParmar VGupta SKadam RShetty OPantvaidya GHawaldar RKalkar PKrishnan NMNair NPanda BVanmali VDesai SGera PChitra JGurav MBhatia DGardi NJoshi SChaubal RBadwe RfalseSurgical Tumor Resection Deregulates Hallmarks of Cancer in Resected Tissue and the Surrounding Microenvironment.Surgery exposes tumor tissue to severe hypoxia and mechanical stress leading to rapid gene expression changes in the tumor and its microenvironment, which remain poorly characterized. We biopsied tumor and adjacent normal tissues from patients with breast (n = 81) and head/neck squamous cancers (HNSC; n = 10) at the beginning (A), during (B), and end of surgery (C). Tumor/normal RNA from 46/81 patients with breast cancer was subjected to mRNA-Seq using Illumina short-read technology, and from nine patients with HNSC to whole-transcriptome microarray with Illumina BeadArray. Pathways and genes involved in 7 of 10 known cancer hallmarks, namely, tumor-promoting inflammation (TNF-A, NFK-B, IL18 pathways), activation of invasion and migration (various extracellular matrix-related pathways, cel2024-01-01T00:00:00Z2024 Jun2026-06-02T10:35:00.753Z2026-05-27T03:07:44.134ZS-EPMC111485423839414910.1158/1541-7786.MCR-23-0265