biostudies-literatureKlein ADNCI NIH HHSar70https://www.ebi.ac.uk/biostudies/studies/S-EPMC11151102biostudies-literatureUnknown35(5)Lysosome turnover and biogenesis are induced in response to treatment of cells with agents that cause membrane rupture, but whether other stress conditions engage similar homeostatic mechanisms is not well understood. Recently we described a form of selective turnover of lysosomes that is induced by metabolic stress or by treatment of cells with ionophores or lysosomotropic agents, involving the formation of intraluminal vesicles within intact organelles through microautophagy. Selective turnover involves noncanonical autophagy and the lipidation of LC3 onto lysosomal membranes, as well as the autophagy gene-dependent formation of intraluminal vesicles. Here, we find a form of microautophagy induction that requires activity of the lipid kinase PIKfyve and is associated with the nuclear traMolecular biology of the cellStress-induced microautophagy is coordinated with lysosome biogenesis and regulated by PIKfyve.PMC11151102P30 CA008748R35 CA263846Lee CPetruzzi KLKlein ADOverholtzer MfalseStress-induced microautophagy is coordinated with lysosome biogenesis and regulated by PIKfyve.Lysosome turnover and biogenesis are induced in response to treatment of cells with agents that cause membrane rupture, but whether other stress conditions engage similar homeostatic mechanisms is not well understood. Recently we described a form of selective turnover of lysosomes that is induced by metabolic stress or by treatment of cells with ionophores or lysosomotropic agents, involving the formation of intraluminal vesicles within intact organelles through microautophagy. Selective turnover involves noncanonical autophagy and the lipidation of LC3 onto lysosomal membranes, as well as the autophagy gene-dependent formation of intraluminal vesicles. Here, we find a form of microautophagy induction that requires activity of the lipid kinase PIKfyve and is associated with the nuclear tra2024-01-01T00:00:00Z2024 May2026-05-07T08:51:12.645Z2026-04-07T22:40:03.538ZS-EPMC111511023853641510.1091/mbc.E23-08-0332