{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lyu F"],"funding":["MRC SIR Grant","University of KwaZulu Natal Research grant","NRF","University of KwaZulu-Natal","The Key Research and Development Program of Jiangsu Province","Jiangsu Agricultural Science and Technology Independent Innovation Fund"],"pagination":["359"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11153293"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["108(1)"],"pubmed_abstract":["Vacuum foam drying (VFD) has been shown to improve the thermostability and long-term shelf life of Newcastle Disease Virus (NDV). This study optimized the VFD process to improve the shelf life of NDV at laboratory-scale and then tested the optimized conditions at pilot-scale. The optimal NDV to T5 formulation ratio was determined to be 1:1 or 3:2. Using the 1:1 virus to formulation ratio, the optimal filling volumes were determined to be 13-17% of the vial capacity. The optimized VFD process conditions were determined to be at a shelf temperature of 25℃ with a minimum overall drying time of 44 h. The vaccine samples prepared using these optimized conditions at laboratory-scale exhibited virus titer losses of ≤ 1.0 log<sub>10</sub> with residual moisture content (RMC) below 3%. Furthermore,"],"journal":["Applied microbiology and biotechnology"],"pubmed_title":["Thermostable vacuum foam dried Newcastle disease vaccine: Process optimization and pilot-scale study."],"pmcid":["PMC11153293"],"funding_grant_id":["121275","CX (22) 2016","BE2020336"],"pubmed_authors":["Zhao YH","Lu Y","Zeng MQ","Olaniran A","Lyu F","Zhou MX","Khoza T","Jiao JJ","Deng BH","Hou J","Nyide B","Jie HY","Zuo XX"],"additional_accession":[]},"is_claimable":false,"name":"Thermostable vacuum foam dried Newcastle disease vaccine: Process optimization and pilot-scale study.","description":"Vacuum foam drying (VFD) has been shown to improve the thermostability and long-term shelf life of Newcastle Disease Virus (NDV). This study optimized the VFD process to improve the shelf life of NDV at laboratory-scale and then tested the optimized conditions at pilot-scale. The optimal NDV to T5 formulation ratio was determined to be 1:1 or 3:2. Using the 1:1 virus to formulation ratio, the optimal filling volumes were determined to be 13-17% of the vial capacity. The optimized VFD process conditions were determined to be at a shelf temperature of 25℃ with a minimum overall drying time of 44 h. The vaccine samples prepared using these optimized conditions at laboratory-scale exhibited virus titer losses of ≤ 1.0 log<sub>10</sub> with residual moisture content (RMC) below 3%. Furthermore,","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-05-07T14:12:15.668Z","creation":"2026-04-07T22:51:35.263Z"},"accession":"S-EPMC11153293","cross_references":{"pubmed":["38836885"],"doi":["10.1007/s00253-024-13174-7"]}}