biostudies-literatureUnknown31(6)Huysentruyt JThe T cell population size is stringently controlled before, during, and after immune responses, as improper cell death regulation can result in autoimmunity and immunodeficiency. RIPK1 is an important regulator of peripheral T cell survival and homeostasis. However, whether different peripheral T cell subsets show a differential requirement for RIPK1 and which programmed cell death pathway they engage in vivo remains unclear. In this study, we demonstrate that conditional ablation of Ripk1 in conventional T cells (Ripk1^ΔCD4) causes peripheral T cell lymphopenia, as witnessed by a profound loss of naive CD4⁺, naive CD8⁺, and FoxP3⁺ regulatory T cells. Interestingly, peripheral naive CD8⁺ T cells in Ripk1^ΔCD4 mice appear to undergo a selective pressure to retain RIPK1 expression following activation. Mixed bone marrow chimeras revealed a competitive survival disadvantage for naive, effector, and memory T cells lacking RIPK1. Additionally, tamoxifen-induced deletion of RIPK1 in CD4-expressing cells in adult life confirmed the importance of RIPK1 in post-thymic survival of CD4⁺ T cells. Ripk1^K45A mice showed no change in peripheral T cell subsets, demonstrating that the T cell lymphopenia was due to the scaffold function of RIPK1 rather than to its kinase activity. Enhanced numbers of Ripk1^ΔCD4 naive T cells expressed the proliferation marker Ki-67⁺ despite the peripheral lymphopenia and single-cell RNA sequencing revealed T cell-specific transcriptomic alterations that were reverted by additional caspase-8 deficiency. Furthermore, Ripk1^ΔCD4Casp8 ^ΔCD4 and Ripk1^ΔCD4Tnfr1^-/- double-knockout mice rescued the peripheral T cell lymphopenia, revealing that RIPK1-deficient naive CD4⁺ and CD8⁺ cells and FoxP3⁺ regulatory T cells specifically die from TNF- and caspase-8-mediated apoptosis in vivo. Altogether, our findings emphasize the essential role of RIPK1 as a scaffold in maintaining the peripheral T cell compartment and preventing TNFR1-induced apoptosis.Cell death and differentiation820-832https://www.ebi.ac.uk/biostudies/studies/S-EPMC11164875biostudies-literatureRIPK1 protects naive and regulatory T cells from TNFR1-induced apoptosis.PMC11164875Ruiz Perez MHuysentruyt JFlies KLambrecht BNVerstraeten BTougaard PSteels WVanden Berghe TDivert TVandenabeele PTakahashi NMaelfait JDeclercq WVadi MfalseRIPK1 protects naive and regulatory T cells from TNFR1-induced apoptosis.The T cell population size is stringently controlled before, during, and after immune responses, as improper cell death regulation can result in autoimmunity and immunodeficiency. RIPK1 is an important regulator of peripheral T cell survival and homeostasis. However, whether different peripheral T cell subsets show a differential requirement for RIPK1 and which programmed cell death pathway they engage in vivo remains unclear. In this study, we demonstrate that conditional ablation of Ripk1 in conventional T cells (Ripk1^ΔCD4) causes peripheral T cell lymphopenia, as witnessed by a profound loss of naive CD4⁺, naive CD8⁺, and FoxP3⁺ regulatory T cells. Interestingly, peripheral naive CD8⁺ T cells in Ripk1^ΔCD4 mice appear to undergo a selective pressure to retain RIPK1 expression following activation. Mixed bone marrow chimeras revealed a competitive survival disadvantage for naive, effector, and memory T cells lacking RIPK1. Additionally, tamoxifen-induced deletion of RIPK1 in CD4-expressing cells in adult life confirmed the importance of RIPK1 in post-thymic survival of CD4⁺ T cells. Ripk1^K45A mice showed no change in peripheral T cell subsets, demonstrating that the T cell lymphopenia was due to the scaffold function of RIPK1 rather than to its kinase activity. Enhanced numbers of Ripk1^ΔCD4 naive T cells expressed the proliferation marker Ki-67⁺ despite the peripheral lymphopenia and single-cell RNA sequencing revealed T cell-specific transcriptomic alterations that were reverted by additional caspase-8 deficiency. Furthermore, Ripk1^ΔCD4Casp8 ^ΔCD4 and Ripk1^ΔCD4Tnfr1^-/- double-knockout mice rescued the peripheral T cell lymphopenia, revealing that RIPK1-deficient naive CD4⁺ and CD8⁺ cells and FoxP3⁺ regulatory T cells specifically die from TNF- and caspase-8-mediated apoptosis in vivo. Altogether, our findings emphasize the essential role of RIPK1 as a scaffold in maintaining the peripheral T cell compartment and preventing TNFR1-induced apoptosis.2024-01-01T00:00:00Z2024 Jun2025-04-04T12:47:52.538Z2025-04-04T12:47:52.538ZS-EPMC111648753873485110.1038/s41418-024-01301-w