biostudies-literatureSharma BNIA NIH HHSNINDS NIH HHS2698-2707https://www.ebi.ac.uk/biostudies/studies/S-EPMC11168514biostudies-literatureUnknown19(12)JOURNAL/nrgr/04.03/01300535-202412000-00026/figure1/v/2024-04-08T165401Z/r/image-tiff Gamma-aminobutyric acid (GABA)ergic neurons, the most abundant inhibitory neurons in the human brain, have been found to be reduced in many neurological disorders, including Alzheimer's disease and Alzheimer's disease-related dementia. Our previous study identified the upregulation of microRNA-502-3p (miR-502-3p) and downregulation of GABA type A receptor subunit α-1 in Alzheimer's disease synapses. This study investigated a new molecular relationship between miR-502-3p and GABAergic synapse function. In vitro studies were performed using the mouse hippocampal neuronal cell line HT22 and miR-502-3p agomiRs and antagomiRs. In silico analysis identified multiple binding sites of miR-502-3p at GABA type A reNeural regeneration researchMicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons.PMC11168514R00 AG065645K99 AG065645R01 NS115834Sharma BRodriguez STorres MMGangwani LKumar SfalseMicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons.JOURNAL/nrgr/04.03/01300535-202412000-00026/figure1/v/2024-04-08T165401Z/r/image-tiff Gamma-aminobutyric acid (GABA)ergic neurons, the most abundant inhibitory neurons in the human brain, have been found to be reduced in many neurological disorders, including Alzheimer's disease and Alzheimer's disease-related dementia. Our previous study identified the upregulation of microRNA-502-3p (miR-502-3p) and downregulation of GABA type A receptor subunit α-1 in Alzheimer's disease synapses. This study investigated a new molecular relationship between miR-502-3p and GABAergic synapse function. In vitro studies were performed using the mouse hippocampal neuronal cell line HT22 and miR-502-3p agomiRs and antagomiRs. In silico analysis identified multiple binding sites of miR-502-3p at GABA type A re2024-01-01T00:00:00Z2024 Dec2026-07-04T03:14:04.703Z2026-07-04T03:11:00.697ZS-EPMC111685143859528810.4103/NRR.NRR-D-23-01064