{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang Z"],"funding":["National Natural Science Foundation of China"],"pagination":["e181"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11170971"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["3(2)"],"pubmed_abstract":["<i>Lactobacillus rhamnosus</i> GG (LGG), the well-characterized human-derived probiotic strain, possesses excellent properties in the maintenance of intestinal homeostasis, immunoregulation and defense against gastrointestinal pathogens in mammals. Here, we demonstrate that the SpaC pilin of LGG causes intestinal epithelium injury by inducing cell pyroptosis and gut microbial dysbiosis in zebrafish. Dietary SpaC activates Caspase-3-GSDMEa pathways in the intestinal epithelium, promotes intestinal pyroptosis and increases lipopolysaccharide (LPS)-producing gut microbes in zebrafish. The increased LPS subsequently activates Gaspy2-GSDMEb pyroptosis pathway. Further analysis reveals the Caspase-3-GSDMEa pyroptosis is initiated by the species-specific recognition of SpaC by TLR4ba, which accou"],"journal":["iMeta"],"pubmed_title":["<i>Lactobacillus rhamnosus</i> GG triggers intestinal epithelium injury in zebrafish revealing host dependent beneficial effects."],"pmcid":["PMC11170971"],"funding_grant_id":["32330110","32172991","31925038"],"pubmed_authors":["Yang HW","Meng DL","Hao Q","Zhou ZG","Meindert de Vos W","Zhang HL","Yang DH","Guan LL","Yao YY","Teame T","Ran C","Zhang Z","Liu SB","Gao CC","Ding QW","Yang YL"],"additional_accession":[]},"is_claimable":false,"name":"<i>Lactobacillus rhamnosus</i> GG triggers intestinal epithelium injury in zebrafish revealing host dependent beneficial effects.","description":"<i>Lactobacillus rhamnosus</i> GG (LGG), the well-characterized human-derived probiotic strain, possesses excellent properties in the maintenance of intestinal homeostasis, immunoregulation and defense against gastrointestinal pathogens in mammals. Here, we demonstrate that the SpaC pilin of LGG causes intestinal epithelium injury by inducing cell pyroptosis and gut microbial dysbiosis in zebrafish. Dietary SpaC activates Caspase-3-GSDMEa pathways in the intestinal epithelium, promotes intestinal pyroptosis and increases lipopolysaccharide (LPS)-producing gut microbes in zebrafish. The increased LPS subsequently activates Gaspy2-GSDMEb pyroptosis pathway. Further analysis reveals the Caspase-3-GSDMEa pyroptosis is initiated by the species-specific recognition of SpaC by TLR4ba, which accou","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Apr","modification":"2026-07-15T08:21:02.959Z","creation":"2026-07-01T03:12:05.84Z"},"accession":"S-EPMC11170971","cross_references":{"pubmed":["38882496"],"doi":["10.1002/imt2.181"]}}