{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["108(1)"],"submitter":["Lin YC"],"pubmed_abstract":["Recombinant adeno-associated virus (rAAV) is a major gene delivery vehicle. We have constructed a stable rAAV producer cell line by integrating essential rAAV genome, viral and helper genes into the genome of HEK293 cell under the control of inducible promoters. Upon induction, the cell line produces transducing rAAV. To gain insight into enhancing rAAV productivity and vector quality, we performed a comparative transcriptomic and proteomic analysis of our synthetic cell line GX2 and two wild-type AAV (wtAAV) production systems, one by virus co-infection and the other by multi-plasmid transfection. The three systems had different kinetics in viral component synthesis but generated comparable copies of AAV genomes; however, the capsid titer of GX2 was an order of magnitude lower compared to"],"journal":["Applied microbiology and biotechnology"],"pagination":["385"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11186941"],"repository":["biostudies-literature"],"pubmed_title":["Comparative transcriptomic and proteomic kinetic analysis of adeno-associated virus production systems."],"pmcid":["PMC11186941"],"pubmed_authors":["Lu M","Lin YC","Hu WS","Cai W"],"additional_accession":[]},"is_claimable":false,"name":"Comparative transcriptomic and proteomic kinetic analysis of adeno-associated virus production systems.","description":"Recombinant adeno-associated virus (rAAV) is a major gene delivery vehicle. We have constructed a stable rAAV producer cell line by integrating essential rAAV genome, viral and helper genes into the genome of HEK293 cell under the control of inducible promoters. Upon induction, the cell line produces transducing rAAV. To gain insight into enhancing rAAV productivity and vector quality, we performed a comparative transcriptomic and proteomic analysis of our synthetic cell line GX2 and two wild-type AAV (wtAAV) production systems, one by virus co-infection and the other by multi-plasmid transfection. The three systems had different kinetics in viral component synthesis but generated comparable copies of AAV genomes; however, the capsid titer of GX2 was an order of magnitude lower compared to","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-04-30T21:22:22.726Z","creation":"2025-04-20T02:16:08.13Z"},"accession":"S-EPMC11186941","cross_references":{"pubmed":["38896252"],"doi":["10.1007/s00253-024-13203-5"]}}