biostudies-literatureDalton GDEunice Kennedy Shriver National Institute of Child Health and Human Development of the National Institutes of HealthAutism Speaks14https://www.ebi.ac.uk/biostudies/studies/S-EPMC11188533biostudies-literatureUnknown20(1)<h4>Background</h4>Autism Spectrum Disorder (ASD) is a group of neurodevelopmental disorders with higher incidence in males and is characterized by atypical verbal/nonverbal communication, restricted interests that can be accompanied by repetitive behavior, and disturbances in social behavior. This study investigated brain mechanisms that contribute to sociability deficits and sex differences in an ASD animal model.<h4>Methods</h4>Sociability was measured in C58/J and C57BL/6J mice using the 3-chamber social choice test. Bulk RNA-Seq and snRNA-Seq identified transcriptional changes in C58/J and C57BL/6J amygdala within which DMRseq was used to measure differentially methylated regions in amygdala.<h4>Results</h4>C58/J mice displayed divergent social strata in the 3-chamber test. TranscriptBehavioral and brain functions : BBFTranscriptome analysis identifies an ASD-Like phenotype in oligodendrocytes and microglia from C58/J amygdala that is dependent on sex and sociability.PMC11188533R01-HD-0880010136Siecinski SKNikolova VDHornburg KJMoy SSGregory SGCofer GPQi YDalton GDJohnson GAJiang YHfalseTranscriptome analysis identifies an ASD-Like phenotype in oligodendrocytes and microglia from C58/J amygdala that is dependent on sex and sociability.<h4>Background</h4>Autism Spectrum Disorder (ASD) is a group of neurodevelopmental disorders with higher incidence in males and is characterized by atypical verbal/nonverbal communication, restricted interests that can be accompanied by repetitive behavior, and disturbances in social behavior. This study investigated brain mechanisms that contribute to sociability deficits and sex differences in an ASD animal model.<h4>Methods</h4>Sociability was measured in C58/J and C57BL/6J mice using the 3-chamber social choice test. Bulk RNA-Seq and snRNA-Seq identified transcriptional changes in C58/J and C57BL/6J amygdala within which DMRseq was used to measure differentially methylated regions in amygdala.<h4>Results</h4>C58/J mice displayed divergent social strata in the 3-chamber test. Transcript2024-01-01T00:00:00Z2024 Jun2025-04-04T12:48:21.232Z2025-04-04T12:48:21.232ZS-EPMC111885333889850210.1186/s12993-024-00240-3