biostudies-literatureCerrone FScience Foundation IrelandUniversity College Dublin377https://www.ebi.ac.uk/biostudies/studies/S-EPMC11189342biostudies-literatureUnknown108(1)Submerged cultivation of edible filamentous fungi (Agaricomycetes) in bioreactors enables maximum mass transfer of nutrients and has the potential to increase the volumetric productivity of fungal biomass compared to solid state cultivation. These aspects are paramount if one wants to increase the range of bioactives (e.g. glucans) in convenient time frames. In this study, Trametes versicolor (M9911) outperformed four other Agaricomycetes tested strains (during batch cultivations in an airlift bioreactor). This strain was therefore further tested in semi-continuous cultivation. Continuous and semi-continuous cultivations (driven by the dilution rate, D) are the preferred bioprocess strategies for biomass production. We examined the semi-continuous cultivation of T. versicolor at dilution rApplied microbiology and biotechnologyAirlift bioreactor-based strategies for prolonged semi-continuous cultivation of edible Agaricomycetes.PMC1118934216/RC/3889O'Connor KELochlainn COCerrone FCallaghan TMcDonald PfalseAirlift bioreactor-based strategies for prolonged semi-continuous cultivation of edible Agaricomycetes.Submerged cultivation of edible filamentous fungi (Agaricomycetes) in bioreactors enables maximum mass transfer of nutrients and has the potential to increase the volumetric productivity of fungal biomass compared to solid state cultivation. These aspects are paramount if one wants to increase the range of bioactives (e.g. glucans) in convenient time frames. In this study, Trametes versicolor (M9911) outperformed four other Agaricomycetes tested strains (during batch cultivations in an airlift bioreactor). This strain was therefore further tested in semi-continuous cultivation. Continuous and semi-continuous cultivations (driven by the dilution rate, D) are the preferred bioprocess strategies for biomass production. We examined the semi-continuous cultivation of T. versicolor at dilution r2024-01-01T00:00:00Z2024 Jun2026-05-07T14:10:59.585Z2026-04-07T22:51:04.59ZS-EPMC111893423888863810.1007/s00253-024-13220-4