biostudies-literatureSengupta ATexas A&M University SystemMIRA Institute, University of TwenteNational Institute of General Medical SciencesNIGMS NIH HHSNational Science Foundation117137https://www.ebi.ac.uk/biostudies/studies/S-EPMC11192522biostudies-literatureUnknown494Increasing studies associating glycerophospholipids with various pathological conditions highlight the need for their thorough characterization. However, the intricate composition of the lipidome due to the presence of lipid isomers poses significant challenges to structural lipidomics. This study uses the anodic corrosion of two metals in a single theta nESI emitter as a tool to simultaneously characterize lipids at multiple isomer levels. Anodic corrosion of cobalt and copper in the positive ion mode generates the metal-adducted lipid complexes, [M+Co]²⁺ and [M+Cu]⁺, respectively. Optimization of parameters such as the distances of the electrodes from the nESI tip allowed the achievement of the formation of one metal-adducted lipid product at a time. Collision-inducInternational journal of mass spectrometryDual Metal Electrolysis in Theta Capillary for Lipid Analysis.PMC111925222139772A-2089R35 GM143047R35GM143047Edwards MEYan XSengupta AfalseDual Metal Electrolysis in Theta Capillary for Lipid Analysis.Increasing studies associating glycerophospholipids with various pathological conditions highlight the need for their thorough characterization. However, the intricate composition of the lipidome due to the presence of lipid isomers poses significant challenges to structural lipidomics. This study uses the anodic corrosion of two metals in a single theta nESI emitter as a tool to simultaneously characterize lipids at multiple isomer levels. Anodic corrosion of cobalt and copper in the positive ion mode generates the metal-adducted lipid complexes, [M+Co]²⁺ and [M+Cu]⁺, respectively. Optimization of parameters such as the distances of the electrodes from the nESI tip allowed the achievement of the formation of one metal-adducted lipid product at a time. Collision-induc2023-01-01T00:00:00Z2023 Dec2026-03-18T14:11:46.528Z2025-04-20T02:19:41.173ZS-EPMC111925223891147910.1016/j.ijms.2023.117137