{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li Y"],"funding":["NHLBI NIH HHS","NCI NIH HHS","NIGMS NIH HHS","NIH HHS"],"pagination":["2320-2336.e6"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11196006"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["84(12)"],"pubmed_abstract":["2'-O-methylation (Nm) is a prominent RNA modification well known in noncoding RNAs and more recently also found at many mRNA internal sites. However, their function and base-resolution stoichiometry remain underexplored. Here, we investigate the transcriptome-wide effect of internal site Nm on mRNA stability. Combining nanopore sequencing with our developed machine learning method, NanoNm, we identify thousands of Nm sites on mRNAs with a single-base resolution. We observe a positive effect of FBL-mediated Nm modification on mRNA stability and expression level. Elevated FBL expression in cancer cells is associated with increased expression levels for 2'-O-methylated mRNAs of cancer pathways, implying the role of FBL in post-transcriptional regulation. Lastly, we find that FBL-mediated 2'-O-methylation connects to widespread 3' UTR shortening, a mechanism that globally increases RNA stability. Collectively, we demonstrate that FBL-mediated Nm modifications at mRNA internal sites regulate gene expression by enhancing mRNA stability."],"journal":["Molecular cell"],"pubmed_title":["2'-O-methylation at internal sites on mRNA promotes mRNA stability."],"pmcid":["PMC11196006"],"funding_grant_id":["R01 HL155632","P50 CA180995","S10 OD025194","R01 GM138407","R01 HL133254","S10 OD025120","R01 CA278832","R01 HL148338","R01 CA256741","P30 CA060553","P41 GM108569","R01 GM125632","R01 CA208257"],"pubmed_authors":["Gao B","Li Y","Yi Y","Chen K","Wang R","Zhang Y","Gao X","Wang X","Zhao D","Zhang L","Zhang LS","Cao Q"],"additional_accession":[]},"is_claimable":false,"name":"2'-O-methylation at internal sites on mRNA promotes mRNA stability.","description":"2'-O-methylation (Nm) is a prominent RNA modification well known in noncoding RNAs and more recently also found at many mRNA internal sites. However, their function and base-resolution stoichiometry remain underexplored. Here, we investigate the transcriptome-wide effect of internal site Nm on mRNA stability. Combining nanopore sequencing with our developed machine learning method, NanoNm, we identify thousands of Nm sites on mRNAs with a single-base resolution. We observe a positive effect of FBL-mediated Nm modification on mRNA stability and expression level. Elevated FBL expression in cancer cells is associated with increased expression levels for 2'-O-methylated mRNAs of cancer pathways, implying the role of FBL in post-transcriptional regulation. Lastly, we find that FBL-mediated 2'-O-methylation connects to widespread 3' UTR shortening, a mechanism that globally increases RNA stability. Collectively, we demonstrate that FBL-mediated Nm modifications at mRNA internal sites regulate gene expression by enhancing mRNA stability.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-06-02T01:13:57.021Z","creation":"2026-05-24T03:07:53.329Z"},"accession":"S-EPMC11196006","cross_references":{"pubmed":["38906115"],"doi":["10.1016/j.molcel.2024.04.011"]}}