{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["3(5)"],"submitter":["Shu X"],"pubmed_abstract":["The integration of RNA metabolic labelling by nucleoside analogues with high-throughput RNA sequencing has been harnessed to study RNA dynamics. The immunoprecipitation purification or chemical pulldown technique is generally required to enrich the analogue-labelled RNAs. Here we developed an a<sup>6</sup>A-seq method, which takes advantage of <i>N<sup>6</sup>-</i>allyladenosine (a<sup>6</sup>A) metabolic labelling on cellular mRNAs and profiles them in an immunoprecipitation-free and mutation-based manner. a<sup>6</sup>A plays a role as a chemical sequencing tag in that the iodination of a<sup>6</sup>A in mRNAs results in 1,<i>N</i> <sup>6</sup>-cyclized adenosine (cyc-A), which induces base misincorporation during RNA reverse transcription, thus making a<sup>6</sup>A-labelled mRNAs detec"],"journal":["Fundamental research"],"pagination":["657-664"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11197751"],"repository":["biostudies-literature"],"pubmed_title":["a<sup>6</sup>A-seq: <i>N</i> <sup>6</sup>-allyladenosine-based cellular messenger RNA metabolic labelling and sequencing."],"pmcid":["PMC11197751"],"pubmed_authors":["Huang C","Liu J","Li T","Cao J","Shu X"],"additional_accession":[]},"is_claimable":false,"name":"a<sup>6</sup>A-seq: <i>N</i> <sup>6</sup>-allyladenosine-based cellular messenger RNA metabolic labelling and sequencing.","description":"The integration of RNA metabolic labelling by nucleoside analogues with high-throughput RNA sequencing has been harnessed to study RNA dynamics. The immunoprecipitation purification or chemical pulldown technique is generally required to enrich the analogue-labelled RNAs. Here we developed an a<sup>6</sup>A-seq method, which takes advantage of <i>N<sup>6</sup>-</i>allyladenosine (a<sup>6</sup>A) metabolic labelling on cellular mRNAs and profiles them in an immunoprecipitation-free and mutation-based manner. a<sup>6</sup>A plays a role as a chemical sequencing tag in that the iodination of a<sup>6</sup>A in mRNAs results in 1,<i>N</i> <sup>6</sup>-cyclized adenosine (cyc-A), which induces base misincorporation during RNA reverse transcription, thus making a<sup>6</sup>A-labelled mRNAs detec","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Sep","modification":"2026-06-01T11:30:52.641Z","creation":"2024-10-15T14:33:25.092Z"},"accession":"S-EPMC11197751","cross_references":{"pubmed":["38933292"],"doi":["10.1016/j.fmre.2023.04.010"]}}