{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Guo X"],"funding":["U.S. Department of Health and Human Services","NINDS NIH HHS","National Institutes of Health"],"pagination":["114192"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11197849"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["43(5)"],"pubmed_abstract":["The preoptic area of the hypothalamus (POA) is essential for sleep regulation. However, the cellular makeup of the POA is heterogeneous, and the molecular identities of the sleep-promoting cells remain elusive. To address this question, this study compares mice during recovery sleep following sleep deprivation to mice allowed extended sleep. Single-nucleus RNA sequencing (single-nucleus RNA-seq) identifies one galanin inhibitory neuronal subtype that shows upregulation of rapid and delayed activity-regulated genes during recovery sleep. This cell type expresses higher levels of growth hormone receptor and lower levels of estrogen receptor compared to other galanin subtypes. single-nucleus RNA-seq also reveals cell-type-specific upregulation of purinergic receptor (P2ry14) and serotonin rec"],"journal":["Cell reports"],"pubmed_title":["Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery from sleep deprivation."],"pmcid":["PMC11197849"],"funding_grant_id":["R21NS122370","R21 NS122370"],"pubmed_authors":["Lian J","Pack AI","Keenan BT","Reiner BC","Guo X"],"additional_accession":[]},"is_claimable":false,"name":"Single-nucleus RNA-seq identifies one galanin neuronal subtype in mouse preoptic hypothalamus activated during recovery from sleep deprivation.","description":"The preoptic area of the hypothalamus (POA) is essential for sleep regulation. However, the cellular makeup of the POA is heterogeneous, and the molecular identities of the sleep-promoting cells remain elusive. To address this question, this study compares mice during recovery sleep following sleep deprivation to mice allowed extended sleep. Single-nucleus RNA sequencing (single-nucleus RNA-seq) identifies one galanin inhibitory neuronal subtype that shows upregulation of rapid and delayed activity-regulated genes during recovery sleep. This cell type expresses higher levels of growth hormone receptor and lower levels of estrogen receptor compared to other galanin subtypes. single-nucleus RNA-seq also reveals cell-type-specific upregulation of purinergic receptor (P2ry14) and serotonin rec","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2025-04-20T02:15:06.729Z","creation":"2025-04-20T02:15:06.729Z"},"accession":"S-EPMC11197849","cross_references":{"pubmed":["38703367"],"doi":["10.1016/j.celrep.2024.114192"]}}