biostudies-literatureUnknown17Day JL<h4>Introduction</h4>The Anaphase Promoting Complex (APC/C), an E3 ubiquitin ligase, plays a key role in cell cycle control, but it is also thought to operate in postmitotic neurons. Most studies linking APC/C function to neuron biology employed perturbations of the APC/C activators, cell division cycle protein 20 (Cdc20) and Cdc20 homologue 1 (Cdh1). However, multiple lines of evidence indicate that Cdh1 and Cdc20 can function in APC/C-independent contexts, so that the effects of their perturbation cannot strictly be linked to APC/C function.<h4>Methods</h4>We therefore deleted the gene encoding Anaphase Promoting Complex 4 (APC4), a core APC/C component, in neurons cultured from conditional knockout (cKO) mice.<h4>Results</h4>Our data indicate that several previously published substratesFrontiers in molecular neuroscience1352782https://www.ebi.ac.uk/biostudies/studies/S-EPMC11199872biostudies-literatureDeletion of a core APC/C component reveals APC/C function in regulating neuronal USP1 levels and morphology.PMC11199872Day JLTirard MBrose NfalseDeletion of a core APC/C component reveals APC/C function in regulating neuronal USP1 levels and morphology.<h4>Introduction</h4>The Anaphase Promoting Complex (APC/C), an E3 ubiquitin ligase, plays a key role in cell cycle control, but it is also thought to operate in postmitotic neurons. Most studies linking APC/C function to neuron biology employed perturbations of the APC/C activators, cell division cycle protein 20 (Cdc20) and Cdc20 homologue 1 (Cdh1). However, multiple lines of evidence indicate that Cdh1 and Cdc20 can function in APC/C-independent contexts, so that the effects of their perturbation cannot strictly be linked to APC/C function.<h4>Methods</h4>We therefore deleted the gene encoding Anaphase Promoting Complex 4 (APC4), a core APC/C component, in neurons cultured from conditional knockout (cKO) mice.<h4>Results</h4>Our data indicate that several previously published substrates2024-01-01T00:00:00Z20242025-04-18T12:24:12.226Z2024-10-15T14:20:57.597ZS-EPMC111998723893293310.3389/fnmol.2024.1352782