biostudies-literatureLottes ENGeorgia State UniversityNIH HHS1029https://www.ebi.ac.uk/biostudies/studies/S-EPMC11201622biostudies-literatureUnknown13(12)The development of cell-type-specific dendritic arbors is integral to the proper functioning of neurons within their circuit networks. In this study, we examine the regulatory relationship between the cytosolic chaperonin CCT, key insulin pathway genes, and an E3 ubiquitin ligase (Cullin1) in dendritic development. CCT loss of function (LOF) results in dendritic hypotrophy in <i>Drosophila</i> Class IV (CIV) multi-dendritic larval sensory neurons, and CCT has recently been shown to fold components of the TOR (Target of Rapamycin) complex 1 (TORC1) in vitro. Through targeted genetic manipulations, we confirm that an LOF of CCT and the TORC1 pathway reduces dendritic complexity, while overexpression of key TORC1 pathway genes increases the dendritic complexity in CIV neurons. Furthermore, boCellsCCT and Cullin1 Regulate the TORC1 Pathway to Promote Dendritic Arborization in Health and Disease.PMC11201622GM131939NS086082Kenneth W. and Georganne F. Honeycutt Fellowship2CI Neurogenomics FellowshipNS130970Brains & Behavior FellowshipTimmins EALottes ENTran TTete BMatta JCox DNPatel AACiger FBhattacharjee SfalseCCT and Cullin1 Regulate the TORC1 Pathway to Promote Dendritic Arborization in Health and Disease.The development of cell-type-specific dendritic arbors is integral to the proper functioning of neurons within their circuit networks. In this study, we examine the regulatory relationship between the cytosolic chaperonin CCT, key insulin pathway genes, and an E3 ubiquitin ligase (Cullin1) in dendritic development. CCT loss of function (LOF) results in dendritic hypotrophy in <i>Drosophila</i> Class IV (CIV) multi-dendritic larval sensory neurons, and CCT has recently been shown to fold components of the TOR (Target of Rapamycin) complex 1 (TORC1) in vitro. Through targeted genetic manipulations, we confirm that an LOF of CCT and the TORC1 pathway reduces dendritic complexity, while overexpression of key TORC1 pathway genes increases the dendritic complexity in CIV neurons. Furthermore, bo2024-01-01T00:00:00Z2024 Jun2025-06-01T01:39:59.077Z2025-06-01T01:39:59.077ZS-EPMC112016223892065810.3390/cells13121029