biostudies-literatureLi NThe National Key Research and Development Program of ChinaThe Jiangxi Provincial Natural Science Foundation of Youth ProjectNational Natural Science Foundation of ChinaThe National Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaJiangxi Provincial Natural Science Foundation of Youth Project135https://www.ebi.ac.uk/biostudies/studies/S-EPMC11205348biostudies-literatureUnknown14(6)Bacterial extracellular vesicles (bEVs) secreted by Gram-negative bacteria are referred to as outer membrane vesicles (OMVs) because they originate in the outer membrane. OMVs are membrane-coated vesicles 20-250 nm in size. They contain lipopolysaccharide (LPS), peptidoglycan, proteins, lipids, nucleic acids, and other substances derived from their parent bacteria and participate in the transmission of information to host cells. OMVs have broad prospects in terms of potential application in the fields of adjuvants, vaccines, and drug delivery vehicles. Currently, there remains a lack of efficient and convenient methods to isolate OMVs, which greatly limits OMV-related research. In this study, we developed a fast, convenient, and low-cost gradient filtration method to separate OMVs that canMembranesEfficient Isolation of Outer Membrane Vesicles (OMVs) Secreted by Gram-Negative Bacteria via a Novel Gradient Filtration Method.PMC1120534820232BAB2160052022YFA110430482360055Wu MTang MDeng KXin HLi NWang LfalseEfficient Isolation of Outer Membrane Vesicles (OMVs) Secreted by Gram-Negative Bacteria via a Novel Gradient Filtration Method.Bacterial extracellular vesicles (bEVs) secreted by Gram-negative bacteria are referred to as outer membrane vesicles (OMVs) because they originate in the outer membrane. OMVs are membrane-coated vesicles 20-250 nm in size. They contain lipopolysaccharide (LPS), peptidoglycan, proteins, lipids, nucleic acids, and other substances derived from their parent bacteria and participate in the transmission of information to host cells. OMVs have broad prospects in terms of potential application in the fields of adjuvants, vaccines, and drug delivery vehicles. Currently, there remains a lack of efficient and convenient methods to isolate OMVs, which greatly limits OMV-related research. In this study, we developed a fast, convenient, and low-cost gradient filtration method to separate OMVs that can2024-01-01T00:00:00Z2024 Jun2026-06-05T03:21:35.608Z2025-04-26T22:35:49.091ZS-EPMC112053483892150210.3390/membranes14060135