biostudies-literatureRoman VAU.S. Department of EnergyU.S. Department of Energy (DOE)DOD | Strategic Environmental Research and Development ProgramDOD | USAF | AMC | Air Force Office of Scientific ResearchDOD | Strategic Environmental Research and Development Program (SERDP)DOD | USAF | AMC | Air Force Office of Scientific Research (AFOSR)e0169423https://www.ebi.ac.uk/biostudies/studies/S-EPMC11205760biostudies-literatureUnknown90(5)Given the multitude of extracellular enzymes at their disposal, many of which are designed to degrade nature's polymers (lignin, cutin, cellulose, etc.), fungi are adept at targeting synthetic polyesters with similar chemical composition. Microbial-influenced deterioration of xenobiotic polymeric surfaces is an area of interest for material scientists as these are important for the conservation of the underlying structural materials. Here, we describe the isolation and characterization of the <i>Papiliotrema laurentii</i> 5307AH (<i>P. laurentii</i>) cutinase, Plcut1. <i>P. laurentii</i> is basidiomycete yeast with the ability to disperse Impranil-DLN (Impranil), a colloidal polyester polyurethane, in agar plates. To test whether the fungal factor involved in this clearing was a secreted eApplied and environmental microbiologyIdentification and recombinant expression of a cutinase from <i>Papiliotrema laurentii</i> that hydrolyzes natural and synthetic polyesters.PMC11205760DE-AC02-05CH1123112RX14CORWP-1381Yan MGrigoriev IVCrable BRWagner DNBiffinger JCrookes-Goodson WJKelley-Loughnane NHaridas SNg VPangilinan JHung CSRoman VANadeau LJGryganskyi AZelik SSchratz LLipzen ACummings LBarlow DStamps BNa HVaraljay VAfalseIdentification and recombinant expression of a cutinase from <i>Papiliotrema laurentii</i> that hydrolyzes natural and synthetic polyesters.Given the multitude of extracellular enzymes at their disposal, many of which are designed to degrade nature's polymers (lignin, cutin, cellulose, etc.), fungi are adept at targeting synthetic polyesters with similar chemical composition. Microbial-influenced deterioration of xenobiotic polymeric surfaces is an area of interest for material scientists as these are important for the conservation of the underlying structural materials. Here, we describe the isolation and characterization of the <i>Papiliotrema laurentii</i> 5307AH (<i>P. laurentii</i>) cutinase, Plcut1. <i>P. laurentii</i> is basidiomycete yeast with the ability to disperse Impranil-DLN (Impranil), a colloidal polyester polyurethane, in agar plates. To test whether the fungal factor involved in this clearing was a secreted e2024-01-01T00:00:00Z2024 May2026-06-01T09:22:57.744Z2025-04-05T17:57:31.069ZS-EPMC112057603862421910.1128/aem.01694-23