{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang Q"],"funding":["Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan"],"pagination":["14811"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11208498"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Ischemic heart diseases are a major global cause of death, and despite timely revascularization, heart failure due to ischemia-hypoxia reperfusion (IH/R) injury remains a concern. The study focused on the role of Early Growth Response 1 (EGR1) in IH/R-induced apoptosis in human cardiomyocytes (CMs). Human induced pluripotent stem cell (hiPSC)-derived CMs were cultured under IH/R conditions, revealing higher EGR1 expression in the IH/R group through quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting (WB). Immunofluorescence analysis (IFA) showed an increased ratio of cleaved Caspase-3-positive apoptotic cells in the IH/R group. Using siRNA for EGR1 successfully downregulated EGR1, suppressing cleaved Caspase-3-positive apoptotic cell ratio. Bioinformatic analysi"],"journal":["Scientific reports"],"pubmed_title":["miR-124-3p downregulates EGR1 to suppress ischemia-hypoxia reperfusion injury in human iPS cell-derived cardiomyocytes."],"pmcid":["PMC11208498"],"funding_grant_id":["20K09125"],"pubmed_authors":["Minatoya K","Masumoto H","Murata K","Yang Q","Ikeda T"],"additional_accession":[]},"is_claimable":false,"name":"miR-124-3p downregulates EGR1 to suppress ischemia-hypoxia reperfusion injury in human iPS cell-derived cardiomyocytes.","description":"Ischemic heart diseases are a major global cause of death, and despite timely revascularization, heart failure due to ischemia-hypoxia reperfusion (IH/R) injury remains a concern. The study focused on the role of Early Growth Response 1 (EGR1) in IH/R-induced apoptosis in human cardiomyocytes (CMs). Human induced pluripotent stem cell (hiPSC)-derived CMs were cultured under IH/R conditions, revealing higher EGR1 expression in the IH/R group through quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting (WB). Immunofluorescence analysis (IFA) showed an increased ratio of cleaved Caspase-3-positive apoptotic cells in the IH/R group. Using siRNA for EGR1 successfully downregulated EGR1, suppressing cleaved Caspase-3-positive apoptotic cell ratio. Bioinformatic analysi","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2025-04-20T02:15:00.414Z","creation":"2025-04-20T02:15:00.414Z"},"accession":"S-EPMC11208498","cross_references":{"pubmed":["38926457"],"doi":["10.1038/s41598-024-65373-x"]}}