biostudies-literatureLuo JQingdao Municipal Natural Science FoundationCentral Public-interest Scientific Institu-tion Basal Research Fund, CAFSChina Agriculture Research SystemsNational Key Research and Development Program of China273https://www.ebi.ac.uk/biostudies/studies/S-EPMC11209076biostudies-literatureUnknown11(6)<i>Perinereis</i> species are essential benthonic animals in coastal ecosystems and have significant roles as live feed in aquaculture, owing to their high-protein and low-fat nutritional profile. Despite their ecological importance, the viral communities associated with these organisms need to be better understood. In this study, we generated 2.6 × 10⁸ reads using meta-transcriptomic sequencing and <i>de novo</i> assembled 5.3 × 10³ virus-associated contigs. We identified 12 novel RNA viruses from two species, <i>Perinereis aibuhitensis</i> and <i>P. wilsoni</i>, which were classified into four major viral groups: <i>Picobirnaviridae</i>, <i>Marnaviridae</i>, unclassified <i>Picornavirales</i>, and unclassified <i>Bunyavirales</i>. Our findings revealed the hidden diVeterinary sciencesMeta-Transcriptomic Analysis Reveals Novel RNA Viruses in Polychaetes <i>Perinereis</i>.PMC11209076NO. 2023TD42CARS-482023YFD240220023-2-1-181-zyyd-jchMeng FZhang FLuo JHuang JDong XZhou CShi WWang GQiu LfalseMeta-Transcriptomic Analysis Reveals Novel RNA Viruses in Polychaetes <i>Perinereis</i>.<i>Perinereis</i> species are essential benthonic animals in coastal ecosystems and have significant roles as live feed in aquaculture, owing to their high-protein and low-fat nutritional profile. Despite their ecological importance, the viral communities associated with these organisms need to be better understood. In this study, we generated 2.6 × 10⁸ reads using meta-transcriptomic sequencing and <i>de novo</i> assembled 5.3 × 10³ virus-associated contigs. We identified 12 novel RNA viruses from two species, <i>Perinereis aibuhitensis</i> and <i>P. wilsoni</i>, which were classified into four major viral groups: <i>Picobirnaviridae</i>, <i>Marnaviridae</i>, unclassified <i>Picornavirales</i>, and unclassified <i>Bunyavirales</i>. Our findings revealed the hidden di2024-01-01T00:00:00Z2024 Jun2026-04-08T18:53:25.77Z2025-04-05T17:57:47.343ZS-EPMC112090763892202010.3390/vetsci11060273