{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Menschikowski H"],"funding":["Deutsche Forschungsgemeinschaft"],"pagination":["869"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11209133"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(6)"],"pubmed_abstract":["Human cytomegalovirus is a ubiquitous herpesvirus that, while latent in most individuals, poses a great risk to immunocompromised patients. In contrast to directly acting traditional antiviral drugs, such as ganciclovir, we aim to emulate a physiological infection control using T cells. For this, we constructed several bispecific T-cell engager (BiTE) constructs targeting different viral glycoproteins of the murine cytomegalovirus and evaluated them in vitro for their efficacy. To isolate the target specific effect without viral immune evasion, we established stable reporter cell lines expressing the viral target glycoprotein B, and the glycoprotein complexes gN-gM and gH-gL, as well as nano-luciferase (nLuc). First, we evaluated binding capacities using flow cytometry and established kill"],"journal":["Viruses"],"pubmed_title":["Evaluation of Bispecific T-Cell Engagers Targeting Murine Cytomegalovirus."],"pmcid":["PMC11209133"],"funding_grant_id":["project number 401821119/GRK2504"],"pubmed_authors":["Hermann M","Bednar C","Thomas M","Menschikowski H","Ensser A","Kubel S","Cordsmeier A","Bauer L"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of Bispecific T-Cell Engagers Targeting Murine Cytomegalovirus.","description":"Human cytomegalovirus is a ubiquitous herpesvirus that, while latent in most individuals, poses a great risk to immunocompromised patients. In contrast to directly acting traditional antiviral drugs, such as ganciclovir, we aim to emulate a physiological infection control using T cells. For this, we constructed several bispecific T-cell engager (BiTE) constructs targeting different viral glycoproteins of the murine cytomegalovirus and evaluated them in vitro for their efficacy. To isolate the target specific effect without viral immune evasion, we established stable reporter cell lines expressing the viral target glycoprotein B, and the glycoprotein complexes gN-gM and gH-gL, as well as nano-luciferase (nLuc). First, we evaluated binding capacities using flow cytometry and established kill","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 May","modification":"2025-04-04T21:22:40.833Z","creation":"2025-04-04T21:22:40.833Z"},"accession":"S-EPMC11209133","cross_references":{"pubmed":["38932161"],"doi":["10.3390/v16060869"]}}