{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Carter MH"],"funding":["NIDDK NIH HHS","ACL HHS","NIAID NIH HHS","NCIRD CDC HHS","NIH HHS","CDC HHS"],"pagination":["978"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11209247"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(6)"],"pubmed_abstract":["Human rotaviruses exhibit limited tropism and replicate poorly in most cell lines. Attachment protein VP4 is a key rotavirus tropism determinant. Previous studies in which human rotaviruses were adapted to cultured cells identified mutations in VP4. However, most such studies were conducted using only a single human rotavirus genotype. In the current study, we serially passaged 50 human rotavirus clinical specimens representing five of the genotypes most frequently associated with severe human disease, each in triplicate, three to five times in primary monkey kidney cells then ten times in the MA104 monkey kidney cell line. From 13 of the 50 specimens, we obtained 25 rotavirus antigen-positive lineages representing all five genotypes, which tended to replicate more efficiently in MA104 cel"],"journal":["Viruses"],"pubmed_title":["Human Rotaviruses of Multiple Genotypes Acquire Conserved VP4 Mutations during Serial Passage."],"pmcid":["PMC11209247"],"funding_grant_id":["5U01 IP000464-05","1U01 IP00163-01","3U01 IP000464-05S1","5R21AI146698-02","U01 IP000464","3U01 IP001063-01S1","U01IP000464","1U01 IP000464-05S2","P30 DK058404","U01 IP001063","R21 AI146698"],"pubmed_authors":["Gribble J","Halasa NB","Denison MR","Mirza SA","Chappell JD","Ogden KM","Carter MH","Diller JR"],"additional_accession":[]},"is_claimable":false,"name":"Human Rotaviruses of Multiple Genotypes Acquire Conserved VP4 Mutations during Serial Passage.","description":"Human rotaviruses exhibit limited tropism and replicate poorly in most cell lines. Attachment protein VP4 is a key rotavirus tropism determinant. Previous studies in which human rotaviruses were adapted to cultured cells identified mutations in VP4. However, most such studies were conducted using only a single human rotavirus genotype. In the current study, we serially passaged 50 human rotavirus clinical specimens representing five of the genotypes most frequently associated with severe human disease, each in triplicate, three to five times in primary monkey kidney cells then ten times in the MA104 monkey kidney cell line. From 13 of the 50 specimens, we obtained 25 rotavirus antigen-positive lineages representing all five genotypes, which tended to replicate more efficiently in MA104 cel","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-05-21T03:15:17.351Z","creation":"2025-04-04T21:22:31.539Z"},"accession":"S-EPMC11209247","cross_references":{"pubmed":["38932271"],"doi":["10.3390/v16060978"]}}