biostudies-literatureHan RNational Key Research and Development Program of ChinaNational Science Foundation686https://www.ebi.ac.uk/biostudies/studies/S-EPMC11209252biostudies-literatureUnknown12(6)Respiratory syncytial virus (RSV) is a leading cause of severe lower respiratory tract disease of infants and older people. There is an urgent need for safe and effective vaccines against RSV infection. In this study, we analyzed the effects of the immune response and protection with the RSV recombinant G protein extracellular domain (G^ecto) combined with various adjuvants as novel subunit vaccines in mice. All groups receiving RSV G^ecto combined with adjuvants exhibited robust humoral and cellular immunity compared to those receiving an adjuvant alone or inactivated RSV vaccine. The greatest effect was observed in mice receiving G^ecto combined with a CpG ODN + Alum salt adjuvant, resulting in the highest production of neutralizing antibodies against both RVaccinesImmune Responses and Protection Profiles in Mice Induced by Subunit Vaccine Candidates Based on the Extracellular Domain Antigen of Respiratory Syncytial Virus G Protein Combined with Different Adjuvants.PMC112092522022YFC2304100 and 2022YFC230340182041041Bing JHan RLi JShan XTan WWang TZhang XWang DSun SCheng XDeng YfalseImmune Responses and Protection Profiles in Mice Induced by Subunit Vaccine Candidates Based on the Extracellular Domain Antigen of Respiratory Syncytial Virus G Protein Combined with Different Adjuvants.Respiratory syncytial virus (RSV) is a leading cause of severe lower respiratory tract disease of infants and older people. There is an urgent need for safe and effective vaccines against RSV infection. In this study, we analyzed the effects of the immune response and protection with the RSV recombinant G protein extracellular domain (G^ecto) combined with various adjuvants as novel subunit vaccines in mice. All groups receiving RSV G^ecto combined with adjuvants exhibited robust humoral and cellular immunity compared to those receiving an adjuvant alone or inactivated RSV vaccine. The greatest effect was observed in mice receiving G^ecto combined with a CpG ODN + Alum salt adjuvant, resulting in the highest production of neutralizing antibodies against both R2024-01-01T00:00:00Z2024 Jun2026-04-08T18:59:47.425Z2025-02-19T00:17:13.886ZS-EPMC112092523893241410.3390/vaccines12060686