biostudies-literatureWang FCapital’s Funds for Health Improvement and ResearchHigh Level Technical Talent Construction Project of Beijing Municipal Health CommissionCapital's Funds for Health Improvement and Research828https://www.ebi.ac.uk/biostudies/studies/S-EPMC11209268biostudies-literatureUnknown16(6)Recombination events in human adenovirus (HAdV) have led to some new highly pathogenic or infectious types. It is vital to monitor recombinant HAdVs, especially in children with acute respiratory tract infections (ARIs). In the retrospective study, HAdV positive specimens were collected from pediatric patients with ARIs during 2015 to 2021, then typed by sequence analysis of the penton base, hexon and fiber gene sequence. For those with inconsistent typing results, a modified method with species-specific primer sets of a fiber gene sequence was developed to distinguish co-infections of different types from recombinant HAdV infections. Then, plaque assays combined with meta-genomic next-generation sequencing (mNGS) were used to reveal the HAdV genomic characteristics. There were 466 cases pVirusesHigh-Frequency Recombination of Human Adenovirus in Children with Acute Respiratory Tract Infections in Beijing, China.PMC11209268Discipline Leader-02-20CFH, shoufa-2022-1G-1131shoufa 2022-4G-1133CFH, shoufa-2022-1G-1131 and shoufa 2022-4G-1133Discipline Leader -02-20Cao LLiu LWang FHan ZZhou YSun YGuo QQu DChen DDe RZhao LZhu RXu YfalseHigh-Frequency Recombination of Human Adenovirus in Children with Acute Respiratory Tract Infections in Beijing, China.Recombination events in human adenovirus (HAdV) have led to some new highly pathogenic or infectious types. It is vital to monitor recombinant HAdVs, especially in children with acute respiratory tract infections (ARIs). In the retrospective study, HAdV positive specimens were collected from pediatric patients with ARIs during 2015 to 2021, then typed by sequence analysis of the penton base, hexon and fiber gene sequence. For those with inconsistent typing results, a modified method with species-specific primer sets of a fiber gene sequence was developed to distinguish co-infections of different types from recombinant HAdV infections. Then, plaque assays combined with meta-genomic next-generation sequencing (mNGS) were used to reveal the HAdV genomic characteristics. There were 466 cases p2024-01-01T00:00:00Z2024 May2026-07-03T03:20:21.408Z2025-04-04T21:22:31.531ZS-EPMC112092683893212110.3390/v16060828