{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kawasue S"],"funding":["Japan Society for the Promotion of Science"],"pagination":["260"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11209320"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(6)"],"pubmed_abstract":["Tetrodotoxin (TTX) is a representative natural toxin causing pufferfish food poisoning, which is especially prominent in East and Southeast Asia, including Japan. TTX has been analyzed through post-column derivatization high-performance liquid chromatography (HPLC), ion-pair LC-MS(/MS), and hydrophilic interaction liquid chromatography (HILIC)-MS(/MS) as alternatives to the mouse bioassay method. However, post-column derivatization requires a system for online derivatization reactions, and with the ion-pair LC-MS approach, it is difficult to remove residual ion-pair reagents remaining in the equipment. Moreover, HILIC-MS provides poor separation compared to reversed-phase (RP) HPLC and requires a long time to reach equilibration. Therefore, we decided to develop a TTX analytical method usi"],"journal":["Toxins"],"pubmed_title":["Tetrodotoxin Derivatization with a Newly Designed Boron Reagent Leads to Conventional Reversed-Phase Liquid Chromatography."],"pmcid":["PMC11209320"],"funding_grant_id":["KJ2188"],"pubmed_authors":["Kuniyoshi K","Kawasue S","Oshiro N","Uema M"],"additional_accession":[]},"is_claimable":false,"name":"Tetrodotoxin Derivatization with a Newly Designed Boron Reagent Leads to Conventional Reversed-Phase Liquid Chromatography.","description":"Tetrodotoxin (TTX) is a representative natural toxin causing pufferfish food poisoning, which is especially prominent in East and Southeast Asia, including Japan. TTX has been analyzed through post-column derivatization high-performance liquid chromatography (HPLC), ion-pair LC-MS(/MS), and hydrophilic interaction liquid chromatography (HILIC)-MS(/MS) as alternatives to the mouse bioassay method. However, post-column derivatization requires a system for online derivatization reactions, and with the ion-pair LC-MS approach, it is difficult to remove residual ion-pair reagents remaining in the equipment. Moreover, HILIC-MS provides poor separation compared to reversed-phase (RP) HPLC and requires a long time to reach equilibration. Therefore, we decided to develop a TTX analytical method usi","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2025-04-04T21:22:17.021Z","creation":"2025-04-04T21:22:17.021Z"},"accession":"S-EPMC11209320","cross_references":{"pubmed":["38922154"],"doi":["10.3390/toxins16060260"]}}