biostudies-literatureZbircea LEThis research was supported through the project INSPIRED (Innovative Strategies for Prevention, Diagnosis and Therapy of Ragweed Pollen Induced Respiratory Diseases), funded by the National Authority for Scientific Research and Innovation (ANCSI)635https://www.ebi.ac.uk/biostudies/studies/S-EPMC11209568biostudies-literatureUnknown12(6)Currently, allergen-specific immunotherapy (AIT) for ragweed allergy is still based on natural allergen extracts. This study aimed to analyse the ability of four commercially available AIT vaccines (CLUSTOID, TYRO-SIT, POLLINEX Quattro Plus and Diater Depot) regarding their ability to induce IgG antibodies against ragweed pollen allergens in rabbits. Accordingly, the IgG reactivity of AIT-induced rabbit sera was tested for ten different ragweed pollen allergens (Amb a 1, 3, 4, 5, 6, 8, 9, 10, 11 and 12) by an ELISA. Furthermore, the ability of rabbit AIT-specific sera to block allergic patients' IgE binding to relevant ragweed allergens (Amb a 1, 4, 6, 8 and 11) and to inhibit allergen-induced basophil activation was evaluated by an IgE inhibition ELISA and a mediator release assay. Only tVaccinesHeterogenous Induction of Blocking Antibodies against Ragweed Allergen Molecules by Allergen Extract-Based Immunotherapy Vaccines.PMC1120956892/09.09.2016, COP 2014-2020, P_37_747, MySMIS 103663Grijincu MTamas TPPanaitescu CStolz FChen KWValenta RTanasie GBuzan MRCotarca MDHutu IPaunescu VZbircea LEHaidar LBabaev EfalseHeterogenous Induction of Blocking Antibodies against Ragweed Allergen Molecules by Allergen Extract-Based Immunotherapy Vaccines.Currently, allergen-specific immunotherapy (AIT) for ragweed allergy is still based on natural allergen extracts. This study aimed to analyse the ability of four commercially available AIT vaccines (CLUSTOID, TYRO-SIT, POLLINEX Quattro Plus and Diater Depot) regarding their ability to induce IgG antibodies against ragweed pollen allergens in rabbits. Accordingly, the IgG reactivity of AIT-induced rabbit sera was tested for ten different ragweed pollen allergens (Amb a 1, 3, 4, 5, 6, 8, 9, 10, 11 and 12) by an ELISA. Furthermore, the ability of rabbit AIT-specific sera to block allergic patients' IgE binding to relevant ragweed allergens (Amb a 1, 4, 6, 8 and 11) and to inhibit allergen-induced basophil activation was evaluated by an IgE inhibition ELISA and a mediator release assay. Only t2024-01-01T00:00:00Z2024 Jun2025-04-21T20:14:35.85Z2025-04-05T17:58:10.616ZS-EPMC112095683893236410.3390/vaccines12060635