biostudies-literatureMazzucchelli SMinistero dell'Istruzione, dell'Università e della RicercaMinistero dell’Istruzione, dell’Università e della Ricerca220https://www.ebi.ac.uk/biostudies/studies/S-EPMC11210105biostudies-literatureUnknown24(1)<h4>Background</h4>A reliable preclinical model of patient-derived organoids (PDOs) was developed in a case study of a 69-year-old woman diagnosed with breast cancer (BC) to investigate the tumour evolution before and after neoadjuvant chemotherapy and surgery. The results were achieved due to the development of PDOs from tissues collected before (O-PRE) and after (O-POST) treatment.<h4>Methods</h4>PDO cultures were characterized by histology, immunohistochemistry (IHC), transmission electron microscopy (TEM), scanning electron microscopy (SEM), confocal microscopy, flow cytometry, real-time PCR, bulk RNA-seq, single-cell RNA sequencing (scRNA-seq) and drug screening.<h4>Results</h4>Both PDO cultures recapitulated the histological and molecular profiles of the original tissues, and they shCancer cell internationalBreast cancer patient-derived organoids for the investigation of patient-specific tumour evolution.PMC11210105PRIN 2017 ID2017EA2NRTagliaferri BCorsi FCamboni TSignati LGasparini PPelucchi PCastagnoli LBonizzi AFranceschini ACarelli SPupa SMMessa LCifola IMangano EConsolandi CVillani LSevergnini MMazzucchelli SCereda CfalseBreast cancer patient-derived organoids for the investigation of patient-specific tumour evolution.<h4>Background</h4>A reliable preclinical model of patient-derived organoids (PDOs) was developed in a case study of a 69-year-old woman diagnosed with breast cancer (BC) to investigate the tumour evolution before and after neoadjuvant chemotherapy and surgery. The results were achieved due to the development of PDOs from tissues collected before (O-PRE) and after (O-POST) treatment.<h4>Methods</h4>PDO cultures were characterized by histology, immunohistochemistry (IHC), transmission electron microscopy (TEM), scanning electron microscopy (SEM), confocal microscopy, flow cytometry, real-time PCR, bulk RNA-seq, single-cell RNA sequencing (scRNA-seq) and drug screening.<h4>Results</h4>Both PDO cultures recapitulated the histological and molecular profiles of the original tissues, and they sh2024-01-01T00:00:00Z2024 Jun2026-07-15T03:04:53.434Z2025-04-05T17:57:56.082ZS-EPMC112101053892670610.1186/s12935-024-03375-5