biostudies-literatureCorleone GAssociazione Italiana per la Ricerca sul Cancro179https://www.ebi.ac.uk/biostudies/studies/S-EPMC11210131biostudies-literatureUnknown43(1)<h4>Background</h4>Enhancer reprogramming plays a significant role in the heterogeneity of cancer. However, we have limited knowledge about the impact of chromatin remodeling in B-Cell Precursor Acute Lymphoblastic Leukemia (BCP-ALL) patients, and how it affects tumorigenesis and drug response. Our research focuses on investigating the role of enhancers in sustaining oncogenic transformation in children with BCP-ALL.<h4>Methods</h4>We used ATAC-seq to study the accessibility of chromatin in pediatric BCP-ALL at three different stages-onset, remission, and relapse. Using a combination of computational and experimental methods, we were able to analyze the accessibility landscape and focus on the most significant cis-regulatory sites. These sites were then functionally validated through the uJournal of experimental & clinical cancer research : CREnhancer engagement sustains oncogenic transformation and progression of B-cell precursor acute lymphoblastic leukemia.PMC11210131MFAG-2009615255I-CARE2 800924Folgiero VDi Giovenale SCaforio MFanciulli MCortile CCorleone GLocatelli FPitisci AGoeman FDe Nicola FBertaina VSorino CfalseEnhancer engagement sustains oncogenic transformation and progression of B-cell precursor acute lymphoblastic leukemia.<h4>Background</h4>Enhancer reprogramming plays a significant role in the heterogeneity of cancer. However, we have limited knowledge about the impact of chromatin remodeling in B-Cell Precursor Acute Lymphoblastic Leukemia (BCP-ALL) patients, and how it affects tumorigenesis and drug response. Our research focuses on investigating the role of enhancers in sustaining oncogenic transformation in children with BCP-ALL.<h4>Methods</h4>We used ATAC-seq to study the accessibility of chromatin in pediatric BCP-ALL at three different stages-onset, remission, and relapse. Using a combination of computational and experimental methods, we were able to analyze the accessibility landscape and focus on the most significant cis-regulatory sites. These sites were then functionally validated through the u2024-01-01T00:00:00Z2024 Jun2025-04-04T21:21:51.877Z2025-04-04T21:21:51.877ZS-EPMC112101313892685310.1186/s13046-024-03075-y