{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kuznetsova A"],"funding":["RDAR","North Dakota Game and Fish Department","Alberta Innovates Bio Solutions","Genome Alberta","Genome Canada"],"pagination":["10932-10940"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11210205"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["58(25)"],"pubmed_abstract":["Chronic wasting disease (CWD) is a contagious prion disease that affects cervids in North America, Northern Europe, and South Korea. CWD is spread through direct and indirect horizontal transmission, with both clinical and preclinical animals shedding CWD prions in saliva, urine, and feces. CWD particles can persist in the environment for years, and soils may pose a risk for transmission to susceptible animals. Our study presents a sensitive method for detecting prions in the environmental samples of prairie soils. Soils were collected from CWD-endemic regions with high (Saskatchewan, Canada) and low (North Dakota, USA) CWD prevalence. Heat extraction with SDS-buffer, a serial protein misfolding cyclic amplification assay coupled with a real-time quaking-induced conversion assay was used t"],"journal":["Environmental science & technology"],"pubmed_title":["Detection of Chronic Wasting Disease Prions in Prairie Soils from Endemic Regions."],"pmcid":["PMC11210205"],"funding_grant_id":["CWD-RP-2122007_30","CFDA # 15.611","#2022N067RC"],"pubmed_authors":["Bahnson CS","McKenzie D","Aiken JM","Kuznetsova A","Ness A","Moffatt E","Bollinger T","Stasiak I"],"additional_accession":[]},"is_claimable":false,"name":"Detection of Chronic Wasting Disease Prions in Prairie Soils from Endemic Regions.","description":"Chronic wasting disease (CWD) is a contagious prion disease that affects cervids in North America, Northern Europe, and South Korea. CWD is spread through direct and indirect horizontal transmission, with both clinical and preclinical animals shedding CWD prions in saliva, urine, and feces. CWD particles can persist in the environment for years, and soils may pose a risk for transmission to susceptible animals. Our study presents a sensitive method for detecting prions in the environmental samples of prairie soils. Soils were collected from CWD-endemic regions with high (Saskatchewan, Canada) and low (North Dakota, USA) CWD prevalence. Heat extraction with SDS-buffer, a serial protein misfolding cyclic amplification assay coupled with a real-time quaking-induced conversion assay was used t","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2025-04-21T20:21:48.273Z","creation":"2025-04-05T17:57:53.004Z"},"accession":"S-EPMC11210205","cross_references":{"pubmed":["38865602"],"doi":["10.1021/acs.est.4c04633"]}}