biostudies-literatureShi HAgricultural Science and Technology Innovation ProgramDirected Improvement of the Characterized Variety Cuibi-1 for Resistance to Bacterial WiltChinese Academy of Agricultural Sciences1818-1834https://www.ebi.ac.uk/biostudies/studies/S-EPMC11213252biostudies-literatureUnknown195(3)Bacterial wilt severely jeopardizes plant growth and causes enormous economic loss in the production of many crops, including tobacco (Nicotiana tabacum). Here, we first demonstrated that the roots of bacterial wilt-resistant tobacco mutant KCB-1 can limit the growth and reproduction of Ralstonia solanacearum. Secondly, we demonstrated that KCB-1 specifically induced an upregulation of naringenin content in root metabolites and root secretions. Further experiments showed that naringenin can disrupt the structure of R. solanacearum, inhibit the growth and reproduction of R. solanacearum, and exert a controlling effect on bacterial wilt. Exogenous naringenin application activated the resistance response in tobacco by inducing the burst of reactive oxygen species and salicylic acid depositionPlant physiologyNaringenin restricts the colonization and growth of Ralstonia solanacearum in tobacco mutant KCB-1.PMC11213252ASTIP-TRI02JY-11110202201011Jiang JZong HWu SYu WWang WWang XDing ASun YShi HCheng YfalseNaringenin restricts the colonization and growth of Ralstonia solanacearum in tobacco mutant KCB-1.Bacterial wilt severely jeopardizes plant growth and causes enormous economic loss in the production of many crops, including tobacco (Nicotiana tabacum). Here, we first demonstrated that the roots of bacterial wilt-resistant tobacco mutant KCB-1 can limit the growth and reproduction of Ralstonia solanacearum. Secondly, we demonstrated that KCB-1 specifically induced an upregulation of naringenin content in root metabolites and root secretions. Further experiments showed that naringenin can disrupt the structure of R. solanacearum, inhibit the growth and reproduction of R. solanacearum, and exert a controlling effect on bacterial wilt. Exogenous naringenin application activated the resistance response in tobacco by inducing the burst of reactive oxygen species and salicylic acid deposition2024-01-01T00:00:00Z2024 Jun2026-05-29T16:54:05.934Z2026-04-08T05:29:35.449ZS-EPMC112132523857332610.1093/plphys/kiae185