biostudies-literatureUnknown79(7)Altarawneh HMinistry of EducationFudan UniversityUniversity of Mutah<h4>Objectives</h4>MRSA is a major cause of hospital-acquired and community-acquired infections. Treatment options for MRSA are limited because of the rapid development of β-lactam resistance. Combining antibiotics offers an affordable, time-saving, viable and efficient approach for developing novel antimicrobial therapies. Both amoxicillin and cefdinir are oral β-lactams with indications for a wide range of bacterial infections and mild side effects. This study aimed to investigate the in vitro and in vivo efficacy of combining these two β-lactams against MRSA strains.<h4>Methods</h4>Fourteen representative prevalent MRSA strains with diverse sequence types (STs) were tested with a combination of amoxicillin and cefdinir, using chequerboard and time-kill assays. The Galleria mellonella laThe Journal of antimicrobial chemotherapy1677-1682https://www.ebi.ac.uk/biostudies/studies/S-EPMC11215534biostudies-literatureSynergistic bactericidal activity of a novel dual β-lactam combination against methicillin-resistant Staphylococcus aureus.PMC11215534Alharbi MFan YAltarawneh HAlhomra TDerrick JPXia GfalseSynergistic bactericidal activity of a novel dual β-lactam combination against methicillin-resistant Staphylococcus aureus.<h4>Objectives</h4>MRSA is a major cause of hospital-acquired and community-acquired infections. Treatment options for MRSA are limited because of the rapid development of β-lactam resistance. Combining antibiotics offers an affordable, time-saving, viable and efficient approach for developing novel antimicrobial therapies. Both amoxicillin and cefdinir are oral β-lactams with indications for a wide range of bacterial infections and mild side effects. This study aimed to investigate the in vitro and in vivo efficacy of combining these two β-lactams against MRSA strains.<h4>Methods</h4>Fourteen representative prevalent MRSA strains with diverse sequence types (STs) were tested with a combination of amoxicillin and cefdinir, using chequerboard and time-kill assays. The Galleria mellonella la2024-01-01T00:00:00Z2024 Jul2025-04-18T15:28:28.629Z2025-04-07T02:10:51.485ZS-EPMC112155343883159910.1093/jac/dkae165