biostudies-literatureUnknown79(7)Mills ROThe International Society for Antimicrobial Chemotherapy<h4>Objectives</h4>Comprehensive data on the genomic epidemiology of hospital-associated Klebsiella pneumoniae in Ghana are scarce. This study investigated the genomic diversity, antimicrobial resistance patterns, and clonal relationships of 103 clinical K. pneumoniae isolates from five tertiary hospitals in Southern Ghana-predominantly from paediatric patients aged under 5 years (67/103; 65%), with the majority collected from urine (32/103; 31%) and blood (25/103; 24%) cultures.<h4>Methods</h4>We generated hybrid Nanopore-Illumina assemblies and employed Pathogenwatch for genotyping via Kaptive [capsular (K) locus and lipopolysaccharide (O) antigens] and Kleborate (antimicrobial resistance and hypervirulence) and determined clonal relationships using core-genome MLST (cgMLST).<h4>Results<The Journal of antimicrobial chemotherapy1529-1539https://www.ebi.ac.uk/biostudies/studies/S-EPMC11215549biostudies-literatureGenomic diversity and antimicrobial resistance in clinical Klebsiella pneumoniae isolates from tertiary hospitals in Southern Ghana.PMC11215549Dadzie IMorgan JMensah ASpadar AAddy HPKAkwetey SADonkoh IEAmpah EOBaker DJAdade NEQuansah EMwintige PSemanshia PSLe-Viet TOwusu EAmoako EOHolt KEMills ROFoster-Nyarko EfalseGenomic diversity and antimicrobial resistance in clinical Klebsiella pneumoniae isolates from tertiary hospitals in Southern Ghana.<h4>Objectives</h4>Comprehensive data on the genomic epidemiology of hospital-associated Klebsiella pneumoniae in Ghana are scarce. This study investigated the genomic diversity, antimicrobial resistance patterns, and clonal relationships of 103 clinical K. pneumoniae isolates from five tertiary hospitals in Southern Ghana-predominantly from paediatric patients aged under 5 years (67/103; 65%), with the majority collected from urine (32/103; 31%) and blood (25/103; 24%) cultures.<h4>Methods</h4>We generated hybrid Nanopore-Illumina assemblies and employed Pathogenwatch for genotyping via Kaptive [capsular (K) locus and lipopolysaccharide (O) antigens] and Kleborate (antimicrobial resistance and hypervirulence) and determined clonal relationships using core-genome MLST (cgMLST).<h4>Results<2024-01-01T00:00:00Z2024 Jul2026-04-08T15:17:39.552Z2025-04-07T02:11:11.264ZS-EPMC112155493875109310.1093/jac/dkae123