{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Greening C"],"funding":["European Research Council"],"pagination":["3357-3372.e19"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11216029"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["187(13)"],"pubmed_abstract":["Microbial hydrogen (H<sub>2</sub>) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H<sub>2</sub>-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H<sub>2</sub>. Moreover, we identify and characterize remarkable hybrid complexes formed thr"],"journal":["Cell"],"pubmed_title":["Minimal and hybrid hydrogenases are active from archaea."],"pmcid":["PMC11216029"],"funding_grant_id":["803768","947317","101078476","695192"],"pubmed_authors":["Banfield JF","Valentin Alvarado LE","Senger M","Mullen S","Land H","Schoelmerich M","Song J","Cabotaje PR","Mao J","Spang A","West-Roberts J","Grinter R","Greening C","Stairs CW","Klamke MA","Ponce-Toledo RI","Lappan R","Rodrigues-Oliveira T","Milton M","Schleper C","Leung PM","Berggren G"],"additional_accession":[]},"is_claimable":false,"name":"Minimal and hybrid hydrogenases are active from archaea.","description":"Microbial hydrogen (H<sub>2</sub>) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H<sub>2</sub>-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H<sub>2</sub>. Moreover, we identify and characterize remarkable hybrid complexes formed thr","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-05-21T03:17:47.098Z","creation":"2025-04-04T12:20:35.154Z"},"accession":"S-EPMC11216029","cross_references":{"pubmed":["38866018"],"doi":["10.1016/j.cell.2024.05.032"]}}