biostudies-literatureUnknown18(6)Mugo RMEinstein Foundation Berlininternationalization booster -Freie Universtät BerlinDFG Research Training Group 2046<h4>Background</h4>The standard diagnosis of Ascaris lumbricoides and other soil-transmitted helminth (STH) infections relies on the detection of worm eggs by copromicroscopy. However, this method is dependent on worm patency and shows only limited accuracy in low-intensity infection settings. We aimed to decipher the diagnostic accuracy of different antibodies using various Ascaris antigens in reference to copromicroscopy and quantitative PCR (qPCR), four months after national STH preventative chemotherapy among school children in western Kenya.<h4>Methodology</h4>STH infection status of 390 school children was evaluated via copromicroscopy (Kato-Katz and mini-FLOTAC) and qPCR. In parallel, Ascaris-specific antibody profiles against larval and adult worm lysates, and adult worm excretory-PLoS neglected tropical diseasese0012279https://www.ebi.ac.uk/biostudies/studies/S-EPMC11216587biostudies-literatureEvaluation of copromicroscopy, multiplex-qPCR and antibody serology for monitoring of human ascariasis in endemic settings.PMC11216587Ebner FRausch SLandt OMwacharo JMugo RMStrube COdiere MRNjomo DWNdungu FMGichuki PMRaulf MKHartmann SMusimbi ZDfalseEvaluation of copromicroscopy, multiplex-qPCR and antibody serology for monitoring of human ascariasis in endemic settings.<h4>Background</h4>The standard diagnosis of Ascaris lumbricoides and other soil-transmitted helminth (STH) infections relies on the detection of worm eggs by copromicroscopy. However, this method is dependent on worm patency and shows only limited accuracy in low-intensity infection settings. We aimed to decipher the diagnostic accuracy of different antibodies using various Ascaris antigens in reference to copromicroscopy and quantitative PCR (qPCR), four months after national STH preventative chemotherapy among school children in western Kenya.<h4>Methodology</h4>STH infection status of 390 school children was evaluated via copromicroscopy (Kato-Katz and mini-FLOTAC) and qPCR. In parallel, Ascaris-specific antibody profiles against larval and adult worm lysates, and adult worm excretory-2024-01-01T00:00:00Z2024 Jun2025-04-21T16:12:36.752Z2025-04-21T16:12:36.752ZS-EPMC112165873888919010.1371/journal.pntd.0012279