biostudies-literatureLiu JWilhelm Sander-StiftungDeutsche Forschungsgemeinschaft94https://www.ebi.ac.uk/biostudies/studies/S-EPMC11218312biostudies-literatureUnknown29(1)<h4>Background</h4>We have previously identified an unsuspected role for GJB3 showing that the deficiency of this connexin protein induces aneuploidy in human and murine cells and accelerates cell transformation as well as tumor formation in xenograft models. The molecular mechanisms by which loss of GJB3 leads to aneuploidy and cancer initiation and progression remain unsolved.<h4>Methods</h4>GJB3 expression levels were determined by RT-qPCR and Western blot. The consequences of GJB3 knockdown on genome instability were assessed by metaphase chromosome counting, multinucleation of cells, by micronuclei formation and by the determination of spindle orientation. Interactions of GJB3 with α-tubulin and F-actin was analyzed by immunoprecipitation and immunocytochemistry. Consequences of GJB3 Cellular & molecular biology lettersImpairment of α-tubulin and F-actin interactions of GJB3 induces aneuploidy in urothelial cells and promotes bladder cancer cell invasion.PMC11218312EI792/7-12019.038.1GU569/6-1Liu JEiseler TAzoitei AZengerling FWezel FHartmann AGunes CJiang WEckstein MHohwieler MElati MBolenz CWang XStilgenbauer SKleger AJohn AfalseImpairment of α-tubulin and F-actin interactions of GJB3 induces aneuploidy in urothelial cells and promotes bladder cancer cell invasion.<h4>Background</h4>We have previously identified an unsuspected role for GJB3 showing that the deficiency of this connexin protein induces aneuploidy in human and murine cells and accelerates cell transformation as well as tumor formation in xenograft models. The molecular mechanisms by which loss of GJB3 leads to aneuploidy and cancer initiation and progression remain unsolved.<h4>Methods</h4>GJB3 expression levels were determined by RT-qPCR and Western blot. The consequences of GJB3 knockdown on genome instability were assessed by metaphase chromosome counting, multinucleation of cells, by micronuclei formation and by the determination of spindle orientation. Interactions of GJB3 with α-tubulin and F-actin was analyzed by immunoprecipitation and immunocytochemistry. Consequences of GJB3 2024-01-01T00:00:00Z2024 Jul2025-04-04T19:23:37.263Z2025-04-04T19:23:37.263ZS-EPMC112183123895649710.1186/s11658-024-00609-2