biostudies-literatureJi YNational Natural Science Foundation of ChinaScience and Technology Department of Guangxi Zhuang Autonomous Region194https://www.ebi.ac.uk/biostudies/studies/S-EPMC11218317biostudies-literatureUnknown15(1)<h4>Background</h4>Repairation of bone defects remains a major clinical problem. Constructing bone tissue engineering containing growth factors, stem cells, and material scaffolds to repair bone defects has recently become a hot research topic. Nerve growth factor (NGF) can promote osteogenesis of bone marrow mesenchymal stem cells (BMSCs), but the low survival rate of the BMSCs during transplantation remains an unresolved issue. In this study, we investigated the therapeutic effect of BMSCs overexpression of NGF on bone defect by inhibiting pyroptosis.<h4>Methods</h4>The relationship between the low survival rate and pyroptosis of BMSCs overexpressing NGF in localized inflammation of fractures was explored by detecting pyroptosis protein levels. Then, the NGF⁺/BMSCs-NSA-Sca bonStem cell research & therapyAcceleration of bone repairation by BMSCs overexpressing NGF combined with NSA and allograft bone scaffolds.PMC1121831781660366320713092022GXNSFAA10302582160416Lv ZGuo YLi QWang NFu CZhao PGu LBei CLin HJi YChen XWang YMao YfalseAcceleration of bone repairation by BMSCs overexpressing NGF combined with NSA and allograft bone scaffolds.<h4>Background</h4>Repairation of bone defects remains a major clinical problem. Constructing bone tissue engineering containing growth factors, stem cells, and material scaffolds to repair bone defects has recently become a hot research topic. Nerve growth factor (NGF) can promote osteogenesis of bone marrow mesenchymal stem cells (BMSCs), but the low survival rate of the BMSCs during transplantation remains an unresolved issue. In this study, we investigated the therapeutic effect of BMSCs overexpression of NGF on bone defect by inhibiting pyroptosis.<h4>Methods</h4>The relationship between the low survival rate and pyroptosis of BMSCs overexpressing NGF in localized inflammation of fractures was explored by detecting pyroptosis protein levels. Then, the NGF⁺/BMSCs-NSA-Sca bon2024-01-01T00:00:00Z2024 Jul2025-04-21T16:14:38.38Z2025-04-21T16:14:38.38ZS-EPMC112183173895671910.1186/s13287-024-03807-z