biostudies-literatureMelendez-Sanchez DInstituto Mexicano del Seguro Social (IMSS)Instituto Mexicano del Seguro Sociale0394723https://www.ebi.ac.uk/biostudies/studies/S-EPMC11218462biostudies-literatureUnknown12(7)<i>Clostridioides difficile</i> (<i>C. difficile</i>) is widely distributed in the intestinal tract of humans, animals, and in the environment. It is the most common cause of diarrhea associated with the use of antimicrobials in humans and among the most common healthcare-associated infections worldwide. Its pathogenesis is mainly due to the production of toxin A (TcdA), toxin B (TcdB), and a binary toxin (CDT), whose genetic variants may be associated with disease severity. We studied genetic diversity in 39 <i>C</i>. <i>difficile</i> isolates from adults and children attended at two Mexican hospitals, using different gene and genome typing methods and investigated their association with <i>in vitro</i> expression of toxins. Whole-genome sequencing in 39 toxigenic <i>C. difficile</i> isolMicrobiology spectrumGenomic and phenotypic studies among <i>Clostridioides difficile</i> isolates show a high prevalence of clade 2 and great diversity in clinical isolates from Mexican adults and children with healthcare-associated diarrhea.PMC11218462FIS/IMSS/PROT/PRIO/16/0and Proyecto 2022 de Reactivación de Protocolos59Camorlinga-Ponce MHernandez LTorres JFlores-Luna LMelendez-Sanchez DAres MMendez Tenorio AfalseGenomic and phenotypic studies among <i>Clostridioides difficile</i> isolates show a high prevalence of clade 2 and great diversity in clinical isolates from Mexican adults and children with healthcare-associated diarrhea.<i>Clostridioides difficile</i> (<i>C. difficile</i>) is widely distributed in the intestinal tract of humans, animals, and in the environment. It is the most common cause of diarrhea associated with the use of antimicrobials in humans and among the most common healthcare-associated infections worldwide. Its pathogenesis is mainly due to the production of toxin A (TcdA), toxin B (TcdB), and a binary toxin (CDT), whose genetic variants may be associated with disease severity. We studied genetic diversity in 39 <i>C</i>. <i>difficile</i> isolates from adults and children attended at two Mexican hospitals, using different gene and genome typing methods and investigated their association with <i>in vitro</i> expression of toxins. Whole-genome sequencing in 39 toxigenic <i>C. difficile</i> isol2024-01-01T00:00:00Z2024 Jul2026-07-15T05:15:40.441Z2024-11-09T15:15:59.635ZS-EPMC112184623886467010.1128/spectrum.03947-23