biostudies-literatureTejeda-Garibay Sinternal Lawrence National Livermore Directed Research and Development fundsNHLBI NIH HHSRuth L. Kirschstein National Research Service Award Individual Predoctoral Fellowship to Promote Diversity in Health-Related ResearchASUCM Academic Affairs Fellowships and Undergraduate Research Symposium (FURS)The American Association of Immunologists Intersect Fellowship Program for Computational Scientists and Immunologists fellowshipUniversity of California Office of the President AwardsASUCM Academic Affairs Fellowships and Undergraduate Research Symposiume0297823https://www.ebi.ac.uk/biostudies/studies/S-EPMC11218535biostudies-literatureUnknown12(7)Coccidioidomycosis, also known as Valley fever, is a disease caused by the fungal pathogen <i>Coccidioides</i>. Unfortunately, patients are often misdiagnosed with bacterial pneumonia, leading to inappropriate antibiotic treatment. The soil <i>Bacillus subtilis</i>-like species exhibits antagonistic properties against <i>Coccidioides in vitro</i>; however, the antagonistic capabilities of host microbiota against <i>Coccidioides</i> are unexplored. We sought to examine the potential of the tracheal and intestinal microbiomes to inhibit the growth of <i>Coccidioides in vitro</i>. We hypothesized that an uninterrupted lawn of microbiota obtained from antibiotic-free mice would inhibit the growth of <i>Coccidioides,</i> while partial <i>in vitro</i> depletion through antibiotic disk diffusion Microbiology spectrumHost tracheal and intestinal microbiomes inhibit <i>Coccidioides</i> growth <i>in vitro</i>.PMC11218535F31 HL160203MRP-17-454959, VFR-19-63395222-ERD-010F31HL160203Zhao LTejeda-Garibay SAmiri BSindi SSWeilhammer DRHoyer KKPimentel MHum NRLoots GGDiep ALfalseHost tracheal and intestinal microbiomes inhibit <i>Coccidioides</i> growth <i>in vitro</i>.Coccidioidomycosis, also known as Valley fever, is a disease caused by the fungal pathogen <i>Coccidioides</i>. Unfortunately, patients are often misdiagnosed with bacterial pneumonia, leading to inappropriate antibiotic treatment. The soil <i>Bacillus subtilis</i>-like species exhibits antagonistic properties against <i>Coccidioides in vitro</i>; however, the antagonistic capabilities of host microbiota against <i>Coccidioides</i> are unexplored. We sought to examine the potential of the tracheal and intestinal microbiomes to inhibit the growth of <i>Coccidioides in vitro</i>. We hypothesized that an uninterrupted lawn of microbiota obtained from antibiotic-free mice would inhibit the growth of <i>Coccidioides,</i> while partial <i>in vitro</i> depletion through antibiotic disk diffusion 2024-01-01T00:00:00Z2024 Jul2026-04-29T12:10:09.091Z2024-11-09T15:16:46.837ZS-EPMC112185353883276610.1128/spectrum.02978-23