biostudies-literatureUnknown10(11)Wang LAn efficient method was discovered for catalyzing the esterification under air using Novozym 435 to obtain pyridine esters. The following conditions were found to be optimal: 60 mg of Novozyme 435, 5.0 mL of n-hexane, a molar ratio of 2:1 for nicotinic acids (0.4 mmol) to alcohols (0.2 mmol), 0.25 g of molecular sieve 3A, a revolution speed of 150 rpm, a reaction temperature of 50 °C, and reaction time of 48 h. Under nine cycles of Novozym 435, the 80 % yield was consistently obtained. Optimum conditions were used to synthesize 23 pyridine esters, including five novel compounds. Among them, gas chromatography-mass spectrometry-olfactometry (GC-MS-O) showed phenethyl nicotinate (<b>3g</b>), (<i>E</i>)-hex-4-en-1-yl nicotinate (<b>3m</b>), and octyl nicotinate (<b>3n</b>) possessed strong arHeliyone32435https://www.ebi.ac.uk/biostudies/studies/S-EPMC11219360biostudies-literatureEnzymatic synthesis of pyridine heterocyclic compounds and their thermal stability.PMC11219360Zhou MWu ZZhao MWang PZhao QWu GLai MWang LfalseEnzymatic synthesis of pyridine heterocyclic compounds and their thermal stability.An efficient method was discovered for catalyzing the esterification under air using Novozym 435 to obtain pyridine esters. The following conditions were found to be optimal: 60 mg of Novozyme 435, 5.0 mL of n-hexane, a molar ratio of 2:1 for nicotinic acids (0.4 mmol) to alcohols (0.2 mmol), 0.25 g of molecular sieve 3A, a revolution speed of 150 rpm, a reaction temperature of 50 °C, and reaction time of 48 h. Under nine cycles of Novozym 435, the 80 % yield was consistently obtained. Optimum conditions were used to synthesize 23 pyridine esters, including five novel compounds. Among them, gas chromatography-mass spectrometry-olfactometry (GC-MS-O) showed phenethyl nicotinate (<b>3g</b>), (<i>E</i>)-hex-4-en-1-yl nicotinate (<b>3m</b>), and octyl nicotinate (<b>3n</b>) possessed strong ar2024-01-01T00:00:00Z2024 Jun2025-04-18T13:51:26.817Z2025-04-04T12:54:42.107ZS-EPMC112193603896198910.1016/j.heliyon.2024.e32435